活络通脑片对大鼠脑缺血再灌注损伤的治疗作用  

Effect of Huoluo Tongnao tablets on rats with cerebral ischemia/reperfusion

作　　者：林艳霞 张胜杰 耿海局 王权[2] 马仁强[2] 陈健文[2] LIN Yan-xia;ZHANG Sheng-jie;GENG Hai-ju;WANG Quan;MA Ren-qiang;CHEN Jianwen(Shenzhen JYMed Technology Co.,Ltd.,Shenzhen Guangdong 518188;School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006)

机构地区：[1]深圳市健元医药科技有限公司,广东深圳518188 [2]中山大学药学院,广州510006

出　　处：《中南药学》2021年第1期38-43,共6页Central South Pharmacy

基　　金：广东省科技计划(No.2015B020211005)。

摘　　要：目的探究活络通脑片对大鼠脑缺血再灌注损伤的作用。方法取SPF级雄性Wistar大鼠利用栓线法制备大鼠脑缺血再灌注模型,按照Zea Longa的5分制评分标准进行神经病学评分。将1~3分的模型动物随机分为模型对照组,活络通脑片低、中、高剂量组(85、170、340 mg·kg-1)和阳性对照组(银杏叶片26 mg·kg-1),同法另设假手术组。造模24 h后开始灌胃给药,连续给药14 d。观察活络通脑片对神经功能缺损及行为学评分、血小板活化因子(PAF)诱导的血小板聚集、脑源性神经营养因子(BDNF)、碱性成纤维细胞生长因子(bFGF)表达和脑组织病理学的影响。结果与模型对照组相比,活络通脑片可改善神经功能缺损及行为学评分(P<0.05);可抑制血小板聚集(P<0.05),提高脑组织中bFGF和BDNF的表达(P<0.05),并对脑皮质和海马损伤有一定的改善作用。结论活络通脑片对大鼠脑缺血再灌注损伤有较好的治疗作用。Objective To determine the effect of Huoluo Tongnao tablets on rats with cerebral ischemia/reperfusion.Methods SPF male Wistar rats were used to prepare the cerebral ischemia/reperfusion model by suture method.The model rats were scored according to Zea Longa standards.The rats scored 1~3 were divided into a model group,Huoluo Tongnao tablet low (85 mg·kg-~1),medium (170 mg·kg-~1) and high dose (340 mg·kg-~1) group,and the positive group (Ginkgo leaf tablets,26 mg·kg-~1) randomly.The sham group was also designed in the same way.The rats were treated with Huoluo Tongnao tablets or Ginkgo leaf tablets for 14 days via intragastric administration 24 h later.Upon finishing the drug treatment,the effect of Huoluo Tongnao tablets on neurological impairment and behavioral score,platelet aggregation,brain histopathology,brain-derived neurotrophic factor and basic fibroblast growth factor in brain was investigated.Results Compared with the model group,Huoluo Tongnao tablets increased the neurological and behavioral score dramatically (P <0.05),decreaced the platelets significantly (P <0.05) and improved bFGF and BDNF expression in the brain distinctly (P <0.05).Besides,Huoluo Tongnao tablets alleviated the cortex and the hippocampus in the brain.Conclusion Huoluo Tongnao tablets has good effect on rats with cerebral ischemia/reperfusion.

关 键 词：活络通脑片 缺血再灌注 脑卒中 脑源性神经营养因子 碱性成纤维细胞生长因子 

分 类 号：R286[医药卫生—中药学]