人工驯养树鼩精子发生过程中MCM7蛋白的表达  

Expression of MCM7 Protein during the Spermatogenesis of Artificially Domesticated Tree Shrew

作　　者：何光勇 唐健 杨芳 钱忠义 杨榆玲 罗兰 HE Guang-yong;TANG Jian;YANG Fang;QIAN Zhong-yi;YANG Yu-ling;LUO Lan(School of Basic Medical Sciences,Kunming Medical University,Kunming Yunnan 650500,China;Clinical Laboratory,Kunming Maternal and Child Health Care Hospital,Kunming Yunnan 650041,China;Dept.of Medical Genetics and Prenatal Diagnosis,Kunming Maternal and Child Health Hospital,Kunming Yunnan 650031,China)

机构地区：[1]昆明医科大学基础医学院,云南昆明650500 [2]云南省妇幼保健院检验科,云南昆明650051 [3]昆明市妇幼保健院医学遗传与产前诊断科,云南昆明650031

出　　处：《昆明医科大学学报》2021年第1期1-5,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2018FE001(-187);2019FE001(-186)]。

摘　　要：目的探讨MCM7蛋白在人工驯养树鼩精子发生过程中的表达特点及其表达是否存在季节性差异,为以树鼩作为动物模型进行生殖研究提供基础性实验数据。方法对全年12组不同月份的成年人工驯养树鼩睾丸组织进行HE染色及MCM7蛋白的免疫组化检测。结果 1~12月份人工驯养树鼩睾丸组织中,MCM7蛋白在曲精小管中主要表达于精原细胞和部分初级精母细胞,其阳性细胞率在2月份和3月份较全年其他月份均有显著性差异(P <0.05)。结论人工驯养树鼩精子发生过程中,MCM7蛋白可能在精原细胞和初级精母细胞的DNA复制过程中发挥作用,其蛋白表达的活跃程度存在季节性差异。Objective To study the expression characteristics of MCM7 protein during spermatogenesis of artificially domesticated tree shrews and whether there are seasonal differences in its expression,so as to provide basic experimental data for reproductive research using tree shrews as animal models.Methods 12 groups of adult male artificially domesticated tree shrews testis tissues of different months were used as the materials and immunohistochemical detection of MCM7 protein was performed on the paraffin tissue sections.Results MCM7 protein was expressed in spermatogonia and some primary spermatocyte of the testes in artificially domesticated tree shrews.Compared with the other months in the whole year,the positive cell rate of MCM7 protein was significantly different in February and March(P<0.05).Conclusions MCM7 protein may play a role in the DNA replication of spermatogonia and primary spermatocytes during the spermatogenesis of artificially domesticated tree shrews,and there was seasonal difference in the activity of MCM7 protein expression.

关 键 词：人工驯养树鼩 精子发生 MCM7蛋白 

分 类 号：Q954.43[生物学—动物学]