老年冠心病慢性心衰患者血清miR-214 galectin-3表达及其预后相关性研究  

Expressions of Serum MiR-214 and Galectin-3 in Elderly Patients with Chronic Heart Failure of Coronary Heart Disease and Prognostic Correlation

作　　者：侯培培[1] 于倩[1] 矫妮[1] 孟锦[1] 栾波[1] HOU Peipei;YU Qian;JIAO Ni(Liaoning Provincial People's Hospital / People's Hospital of China Medical University, Liaoning Shenyang 110016, China)

机构地区：[1]辽宁省人民医院/中国医科大学人民医院,辽宁沈阳110016

出　　处：《河北医学》2021年第1期45-50,共6页Hebei Medicine

基　　金：辽宁省科学技术计划项目,(编号:2018020281)。

摘　　要：目的:检测老年冠心病慢性心衰患者血清微小RNA-214(miR-214)、半乳糖凝集素-3(galectin-3)水平,并探讨其水平与预后的相关性。方法:选取2017年7月至2019年4月于本院心内科住院治疗的72例老年冠心病慢性心衰患者为心衰组;同期选取健康体检者84例作为对照组;根据出院12个月内是否发生心血管事件将冠心病慢性心衰患者分为心血管事件组23例和非心血管事件组49例。采用实时荧光定量PCR(qRT-PCR)法检测血清miR-214水平;酶联免疫吸附(ELISA)法检测血清galectin-3、氨基N末端B型利钠肽前体(NT-proBNP)水平;心脏超声检查心功能指标左心室射血分数(LVEF)、左室舒张末期内径(LVEDD)及左室收缩末内径(LVESD);分析慢性心衰患者血清miR-214、galectin-3水平与心功能指标相关性;分析影响慢性心衰患者预后的因素。结果:心衰组miR-214、galectin-3、LVESD、LVEDD、NT-proBNP高于对照组,LVEF低于对照组(P<0.05);慢性心衰患者血清中miR-214、galectin-3水平与LVESD、LVEDD、NT-proBNP呈正相关(P<0.05),与LVEF呈负相关(P<0.05);心血管事件组miR-214、galectin-3、LVESD、LVEDD、NT-proBNP高于非心血管事件组,LVEF低于非心血管事件组(P<0.05);miR-214、galectin-3是影响慢性心衰患者发生不良心血管事件的独立危险因素(P<0.05),LVEF是影响慢性心衰患者发生不良心血管事件的保护因素(P<0.05)。结论:miR-214、galectin-3在老年冠心病慢性心衰患者血清中呈高表达,对老年冠心病慢性心衰患者预后预测可能具有重要意义。Objective:To detect the levels of serum microRNA-214(miR-214)and galectin-3 in elderly patients with coronary heart disease and chronic heart failure,and to explore the correlation between their levels and prognosis.Methods:From July 2017 to April 2019,72 elderly patients with chronic heart failure of coronary heart disease in the Department of Cardiology of our hospital were selected as heart failure group;at the same time,84 healthy people were selected as the control group;in addition,according to whether cardiovascular events occurred within 12 months after discharge,patients with chronic heart failure were divided into cardiovascular event group(23 cases)and non cardiovascular event group(49 cases).The level of serum miR-214 was detected by real-time fluorescent quantitative PCR(qRT-PCR);the levels of serum galectin-3 and N-terminal pro-B-type natriuretic peptide(NT-proBNP)were detected by enzyme linked immunosorbent assay(ELISA);left ventricular ejection fraction(LVEF),left ventricular end diastolic diameter(LVEDD)and left ventricular end systolic diameter(LVESD)were measured by echocardiography;the correlation between serum miR-214,galectin-3 and cardiac function indexes in patients with chronic heart failure was analyzed;and the factors influencing the prognosis of patients with chronic heart failure were analyzed.Results:MiR-214,galectin-3,LVESD,LVEDD and NT-proBNP in HF group were higher than those in the control group,and LVEF was lower than that in the control group(P<0.05);the levels of serum miR-214 and galectin-3 were positively correlated with LVESD,LVEDD and NT-proBNP(P<0.05),and negatively correlated with LVEF(P<0.05);miR-214,galectin-3,LVESD,LVEDD and NT-proBNP in cardiovascular event group were higher than those in non cardiovascular event group,and LVEF was lower than those in non cardiovascular event group(P<0.05);miR-214 and galectin-3 were independent risk factors of adverse cardiovascular events in patients with chronic heart failure(P<0.05),and LVEF was the protective factor of adverse c

关 键 词：冠心病慢性心衰 老年人 血清 微小RNA-214 半乳糖凝集素-3 预后 

分 类 号：R54[医药卫生—心血管疾病]