抗原致敏DC联合CTL细胞对小细胞肺癌NCI-H209细胞移植瘤裸鼠的疗效观察  

Treatment efficacy of antigen sensitized DC combined with CTL cells on nude mice bearing small cell lung cancer NCI-H209 cell transplanted tumor

作　　者：彭玉澄 黄艳春[1] 杨佳[1] PENG Yucheng;HUANG Yanchun;YANG Jia(Clinical Laboratory,the Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学附属肿瘤医院检验科,乌鲁木齐830011

出　　处：《新疆医科大学学报》2021年第1期39-43,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金(2016D01C377)。

摘　　要：目的探讨小细胞肺癌NCI-H209细胞裂解物致敏树突细胞(DC)与同源细胞毒性T淋巴细胞(CTL)共培养后的DC-CTL细胞对裸鼠小细胞肺癌移植瘤治疗的有效性。方法建立BALB/c裸鼠小细胞肺癌移植瘤模型,并随机分模型组、CTL组、未致敏DC-CTL组、抗原致敏DC-CTL组,每3天治疗1次,连续6次。观察肿瘤生长情况,绘制移植瘤体积变化曲线。治疗结束后第4天每组随机处死10只裸鼠,测量瘤质量,计算抑瘤率(inhibition ratio,IR),观察肿瘤病例特点。各组剩余裸鼠观察存活期。结果治疗结束后CTL组,DC-CTL组和致敏DC-CTL组裸鼠肿瘤体积和质量均有下降,特别是致敏DC-CTL组较模型组下降明显,抑瘤率达(27.34±6.47)%,差异有统计学意义(P<0.05)。病理切片观察3个治疗组均发现较多的间质炎细胞,以淋巴细胞为主,特别是致敏DC-CTL组,可见大片的肿瘤组织坏死和弥漫成片的淋巴细胞。从生存期观察到,对照组中位生存期(38.00±3.68)d;CTL组中位生存期(44.00±3.16)d;DC-CTL组中位生存期(48.00±3.68)d;抗原致敏DCCTL组中位生存期(50.00±9.85)d。3个治疗组与对照组比较差异有统计学意义(P<0.05),但CTL组与DC-CTL组比较差异无统计学意义,抗原致敏DC-CTL组与其它2个治疗组比较差异有统计学意义(P<0.05)。结论经NCI-H209细胞裂解物致敏的DC-CTL细胞能够明显抑制裸鼠小细胞肺癌移植瘤的生长,延长动物存活期。Objective To investigate the efficacy of DC-CTL cells co-cultured with cytotoxic T lymphocytes(CTL)and sensitized dendritic cells(DC)from nci-h209 cell lysates in the treatment of small cell lung cancer in nude mice.Methods The BALB/c small cell lung cancer transplantation model in nude mice was established and randomly divided into model group,CTL group,unsensitized DC-CTL group and antigen-sensitized DC-CTL group.The tumorgrowth was observed and the tumor volume curve was plotted.Ten nude mice were randomly sacrificed in each group on the 4th day after the treatment,tumor mass was measured,inhibition ratio(IR)was calculated,and tumor case characteristics were observed.Survival of remaining nude mice in each group was observed.Results After the treatment,the tumor volume and mass of nude mice in the CTL group,DC-CTL group and sensitized DC-CTL group all de⁃creased,especially the sensitized DC-CTL group decreased significantly compared with the model group(the tumor in⁃hibition rate was 27.34±6.48%,P<0.05).In the three treatment groups,a large number of stromal cells were found,mainly lymphocytes,especially in the sensitized DC-CTL group.Large areas of tumor tissue necrosis and diffuse lymphocytes were observed.The median survival time of the control group,CTL group,DC-CTL group and sensitized DCCTL group were 38±3.68,44±3.16,48±3.60 and 50±9.85 respectively.There were statistical differences between the three treatment groups and the control group(P<0.05),but there was no difference between the CTL group and the DCCTL group,and there were statistical differences between the antigen-sensitized DC-CTL group and the other two treatment groups(P<0.05).Conclusion The DC-CTL cells sensitized by nci-h209 cell lysates can significantly inhibit the growth of transplanted small-cell lung cancer tumors in nude mice and prolong the survival time of the animals.

关 键 词：小细胞肺癌 树突状细胞-细胞毒性T淋巴细胞 过继性细胞免疫治疗 

分 类 号：R284.1[医药卫生—中药学]