绵羊IGF1基因表达、多态性与毛用性能相关分析  

The IGF1 Gene Expression,Polymorphism and Correlation with Wool Traits Analysis in Sheep

作　　者：罗新惠 张立春[1] 刘艳光 柳俭强 翟博[1] 曹阳[1] 张明新[1] 金海国[1] LUO Xinhui;ZHANG Lichun;LIU Yanguang;LIU Jianqiang;ZAI Bo;CAO Yang;ZHANG Mingxin;JIN Haiguo(Institute of Animal Biotechnology,Jilin Academy of Agricultural Science,Gongzhuling 136100,China;Agricultural College,Yanbian University,Yanji 130021,China)

机构地区：[1]吉林省农业科学院动物生物技术研究所,吉林公主岭136100 [2]延边大学农学院,吉林延吉130021

出　　处：《聊城大学学报（自然科学版）》2021年第2期59-64,共6页Journal of Liaocheng University:Natural Science Edition

基　　金：国家863计划(2013AA102506)资助。

摘　　要：为探讨绵羊胰岛素样生长因子1(Insulin-like growth factor 1,IGF1)基因与毛用性状的关系,本实验首先通过RT-PCR(Reverse Transcript Polymerase Chain Reaction)法从新吉细毛羊皮肤组织中克隆出IGF1基因,并利用ELISA和qRT-PCR(Quantity reverse transcript polymerase chain reaction)法检测比较新吉细毛羊和小尾寒羊外周血和皮肤组织中IGF1表达差异,再利用HRM(High-resolution melting)方法检测IGF1基因在新吉细毛羊、小尾寒羊和陶赛特羊群体中的多态性,最后进行单因素方差分析检验IGF1不同基因型与新吉细毛羊毛用性状的相关性。结果表明绵羊皮肤组织中表达IGF1基因,新吉细毛羊外周血和皮肤组织中的IGF1表达水平略高于小尾寒羊,但差异不显著。PCR测序结果显示存在c.110 C>T和c.153 T>C多态性位点,其中c.110 C>T引起a.37 Ala>Val突变。进一步HRM方法结合测序结果显示新吉细毛羊、小尾寒羊和陶赛特绵羊三个群体中c.153 T>C存在三种基因型(TT、CT、CC),TT纯合型在三个群体中频率最低,且在新吉细毛羊群体内呈现出Hardy-weinberg极度不平衡状态(p<0.0001)。单因素方差分析结果显示TT基因型在拉伸长度和产毛量指标方面高于CC基因型(p<0.05)。绵羊IGF1基因多态性的发现与毛用性状相关分析将为绵羊毛用性状育种实践奠定基础。To explore the correlation of the IGF1 gene with wool trait in sheep.In this study,the IGF1 gene mRNAs were cloned by RT-PCR using skin tissues from Xinji Fine wool sheep as template.The contents ofIGF1 in peripheral blood and skin were measured by ELISA and qRT-PCR respectively.The polymorphism of IGF1 was tested by HRM among Xinji Fine Wool Sheep,Small-tail Han sheep and Dorset Sheep.In the end,the correlation of genotype with wool traits was analyzed by One-way ANOVA in Xinji Fine Wool Sheep population.The RT-PCR showed IGF1 was expressed in skin and there were two SNPs(c.110 C>T and c.153 T>C ) in the ORF region.And the mutant of c.110 C>T caused a.37 Ala >Val mutant in protein.The ELISA and qRT-PCR data approved that Xinji Fine Wool Sheep had higher level IGF1 than Small-tail Han sheep in both serum and skin,but the data didn’t have statistical significance( p > 0.05).The polymorphism test displays the c.110 C>T is fake and three genotypes were constructed by c.153 T>C .and the frequency of TT was smallest in these populations.The Chi-square test showed the frequency of genotypes was in Hardy-Weinberg disequilibrium( p < 0.0001).the TT genotype individual has better staple length and wool yield than CC genotype( p < 0.05).The identification of SNP on IGF1 gene and relationship with wool traits in Xinji Fine Wool sheep can provide the theoretical evidence for fine wool sheep breeding.

关 键 词：IGF1基因 多态性 新吉细毛羊绵羊 毛用性能 高分辨率熔解曲线法 

分 类 号：Q953[生物学—动物学]