蛇毒中生物活性酶的研究进展  

Progress on Bioactive Enzymes in Snake Venom

作　　者：刘秉宜 王庆鹏 王正平 张瑞岩 韩军 LIU Bingyi;WANG Qingpeng;WANG Zhengping;ZHANG Ruiyan;HAN Jun(Institute of biopharmaceutics of LiaoCheng university,LiaoCheng 252059,China;Liaocheng High-Tech Biotechnology Co.Ltd,Liaocheng 252059,China)

机构地区：[1]聊城大学生物制药研究院,山东聊城252059 [2]聊城高新生物技术有限公司,山东聊城252059

出　　处：《聊城大学学报（自然科学版）》2021年第2期81-88,共8页Journal of Liaocheng University:Natural Science Edition

基　　金：国家自然科学基金项目(21807056);山东省科技计划项目(2014GSF118121);山东省自然科学基金项目(ZR2013HZ002);山东省抗体制药协同创新中心开发课题(CIC-AD1834,CIC-AD1829);聊城大学博士及高层次人才科研启动基金项目(318051827,318051738);聊城大学科研基金项目(318011907)资助。

摘　　要：蛇毒是一类包含蛋白质、多肽和金属离子等成份的混合物。蛇毒中的多种生物活性酶决定了其功能的多样性,例如凝血、溶栓、止痛和抗肿瘤作用等。然而,由于蛇粗毒免疫原性强和副作用多等缺点,其临床应用受到了一定的限制。随着新技术和分离纯化方法的发展,越来越多蛇毒产品的新功能被开发出来。针对蛇毒成分的分离纯化技术和分析鉴定方法,对研究较多的蛇毒中存在的活性酶及其生物学功能进行了总结,尤其阐述了蛇毒纤溶酶的种类及其在溶栓作用方面的机制,旨在对蛇毒中生物活性酶药用功能的技术开发进行全面和深入的了解。Snake venom is a kind of mixture including protein,polypeptide and metal ion.The diversity of protease in snake venom determines its multiple functions,such as coagulation,antithrombotic,analgesic and antitumor.However,the clinical applications of crude extracts form snake venom are limited by strong immunogenicity and other side effects.With the development of new technologies and purification methods,more and more new functions of snake venom protease have been developed.In order to further understand the biological functions and the corresponding active mechanisms of the snake venom components,the methods of separation,purification,analysis and identification of snake venom components were reviewed,and the active enzymes which have been detailly studied were summarized,especially the types and mechanism of fibrinolytic enzyme of snake venom.

关 键 词：蛇毒 分离纯化方法 纤溶酶 溶栓 生物活性 

分 类 号：R969[医药卫生—药理学]