宫腔镜术治疗宫腔黏连后患者miR-543、miR-135a表达与再复发关系  

The correlation between the expressions of miR-543 and miR-135a and the recurrence of uterine adhesions of women after hysteroscopic treatment

作　　者：陈国英[1] 邱萍[1] 史淑兰[1] CHEN Guoying;QIU Ping;SHI Shulan(People's Hospital of Jingjiang,Jiangsu Province,214500)

机构地区：[1]江苏省靖江市人民医院,214500

出　　处：《中国计划生育学杂志》2021年第1期138-142,共5页Chinese Journal of Family Planning

摘　　要：目的:探究宫腔黏连宫腔镜术治疗后患者子宫内膜组织中miR-543、miR-135a表达及复发关系。方法:回顾性选取2017年9月-2019年9月本院收治的行宫腔镜术治疗的中、重度宫腔黏连患者78例,根据宫腔镜治疗1年后复发情况分为复发组23例、未复发组55例。采用实时荧光定量PCR法检测患者子宫内膜组织中miR-543、miR-135a表达,Pearson法分析miR-543、miR-135a与SIRT1 mRNA、TGF-βmRNA表达相关性;logistic回归模型分析影响宫腔镜治疗后复发的危险因素;ROC曲线分析miR-543、miR-135a表达对宫腔镜治疗后复发的预测价值。结果:复发组重度黏连、术前月经量过少、术前闭经比例、术前宫腔操作次数及SIRT1 mRNA、TGF-βmRNA水平等均高于未复发组,miR-543、miR-135a表达低于未复发组(均P<0.05)。miR-543、miR-135a均与SIRT1 mRNA、TGF-βmRNA水平呈负相关(P<0.05)。重度黏连、术前宫腔操作次数多、高SIRT1 mRNA水平、高TGF-βmRNA水平、低miR-543水平、低miR-135a水平是影响宫腔镜术治疗后复发的独立危险因素(P<0.05),二者联合预测治疗后复发的曲线下面积为0.856(95%CI:0.771~0.924),敏感度为91.3%,特异度为74.5%。结论:宫腔黏连患者子宫内膜组织中miR-543、miR-135a表达降低,且对宫腔镜术治疗黏连复发有一定评估价值。Objective:To investigate the correlation between the expressions of miR-543 and miR-135a and the recurrence of uterine adhesions of women after hysteroscopic treatment.Methods:From September 2017 to September 2019,78 women with moderate and severe uterine adhesions treated by hysteroscopy were selected and were divided into group A(23 women with recurrence within 1 year after hysteroscopic treatment)and group B(55 women without recurrence).The expression levels of miR-543 and miR-135a were detected by real-time fluorescent quantitative PCR(qRT-PCR),Pearson's method was used to analyze the correlations between the levels of miR-543 and miR-135a and SIRT1 mRNA,TGF-βmRNA expressions,Logistic regression model was used to analyze the risk factors of recurrence of uterine adhesions after hysteroscopic treatment,and ROC curve was used to analyze the values of the expression levels of miR-543 and miR-135a for predicting recurrence of uterine adhesions after hysteroscopic treatment.Results:The proportions of severe adhesion and preoperative hypomenorrhea,and preoperative of amenorrhea,the number of preoperative uterine cavity operation,and the expression levels of SIRT1 mRNA and TGF-βmRNA of women in group A were significant higher than those of women in group B,while the expression levels of miR-543 and miR-135a of women in group A were significant lower(all P<0.05).Both Mir-543 level and Mir-135a level were negatively correlated with SIRT1 mRNA and TGF-mRNA levels(P<0.05).The proportion of severe adhesion,the number of preoperative uterine cavity operation,the high levels of SIRT1 mRNA and TGF-βmRNA,and the low miR-543 and Mir-135a levels were independent risk factors of recurrence after endoscopic treatment(P<0.05).The area under the curve,the sensitivity,and the specificity of combined detection of miR-543 and miR-135a for predicting the recurrence after endoscopic treatment were 0.856(95%CI:0.771-0.924),91.3%,and 74.5%,respectively.Conclusion:The decreasing of the expression levels of miR-543 and miR-135a in endom

关 键 词：宫腔黏连 宫腔镜术 复发 miR-543 miR-135a 

分 类 号：R713.4[医药卫生—妇产科学]