金丝桃素光敏活性对MDA-MB-231细胞的抑制作用  

Inhibitory effect of hypericin photosensitizing activity on human breast cancer MDA-MB-231 cells

作　　者：刘高峰 胡小梅 陈广瑞 LIU Gao-feng(School of Life Science,Bengbu Medical College,Bengbu,Anhui,233030,China)

机构地区：[1]蚌埠医学院生命科学学院,安徽蚌埠233030

出　　处：《齐齐哈尔医学院学报》2020年第19期2377-2380,共4页Journal of Qiqihar Medical University

基　　金：安徽省教育厅自然科学研究重点项目(KJ2018A0993、KJ2017A228);安徽省大学生创新训练项目(201810367020)。

摘　　要：目的探讨光激活的金丝桃素对MDA-MB-231细胞的体外抑制活性及其作用机制。方法MDA-MB-231细胞加入不同浓度(1、2、4和8μM)的金丝桃素,加药1 h后给予照射能量为1.5 J/cm 2的光照照射15 min,分别培养24 h和48 h,CCK8检测细胞活力;通过AO-EB和Hoechst33258染色,观察MDA-MB-231细胞的形态变化;PI染色、流式细胞术检测MDA-MB-231细胞凋亡;DCFH-DA染色、流式细胞仪检测细胞内ROS水平变化。结果光激活后,4μM和8μM浓度的金丝桃素可以有效抑制MDA-MB-231细胞的增殖,诱导其的凋亡;MDA-MB-231细胞内ROS水平显著增加。结论光激活的金丝桃素对MDA-MB-231细胞具有较好的体外抗肿瘤作用。Objective To investigate the inhibitory activity and mechanism of hypericin on human breast cancer MDA-MB-231 cells in vitro under light-induced conditions.Methods Human breast cancer MDA-MB-231 cells were exposed to different concentration of hypericin(1,2,4,and 8μM),then 15 minutes light irradiation(1.5 J/cm 2)was given after an hour.24 h and 48 h later,cell viability was assessed using CCK8 assay.The morphological changes of MDA-MB-231 cells were observed using AO-EB and Hoechst33258 staining.Flow cytometry with PI staining was performed to detect apoptosis of MDA-MB-231 cells.Flow cytometry with DCFH-DA staining was used to detect the ROS levels in MDA-MB-231 cells.Results Photo-activated hypericin at 4μM and 8μM concentrations could effectively inhibit the proliferation and trigger apoptosis of human breast cancer MDA-MB-231 cells,and increased the ROS levels of human breast cancer MDA-MB-231 cells significantly.Conclusions Photo-activated hypericin shows potent anti-tumor activity in vitro against human breast cancer MDA-MB-231 cells.

关 键 词：金丝桃素 光敏活性 人乳腺癌 抑制作用 

分 类 号：R285.5[医药卫生—中药学]