柚苷酶高产菌株选育及其产酶在蜜橘果汁脱苦中的应用  

Mutation of high naringinase producing strains and its application in debittering citrus juice

作　　者：夏辛珂 张媛娥 雷生姣 胡彪 陈钰亭 付彩霞 XIA Xinke;ZHANG Yuane;LEI Shengjiao;HU Biao;CHEN Yuting;FU Caixia(College of Biological and Pharmaceutical,China Three Gorges University,Yichang 443002,China;Research and Development Center,Hubei Tulaohan Flavouring and Food Co.,Ltd.,Yichang 443000,China)

机构地区：[1]三峡大学生物与制药学院,湖北宜昌443002 [2]湖北土老憨生态农业科技股份有限公司,湖北宜昌443000

出　　处：《食品与发酵工业》2021年第2期226-232,共7页Food and Fermentation Industries

基　　金：宜昌市应用基础研究项目(A19-302-02);宜昌海通食品有限公司(SDHZ2016027);宜昌市科学技术研究与开发项目(A18-201);湖北土老憨生态农业科技股份有限公司(SDHZ2017160);三峡大学学位论文培优基金项目资助。

摘　　要：为了得到1株高产柚苷酶菌株并探究其工业应用价值,从发霉的柚子皮上筛选出1株塔宾曲霉(Aspergillus tubingensis)MN589840,采用紫外与常压室温等离子体(atmospheric room temperature plasma,ARTP)复合诱变技术,获得突变株UA13,其柚苷酶活力可达41.524 U/mL,是原始菌株的3.06倍。对酶解条件进行探究,其酶解最适温度为40~50℃,最适pH值为5.0。利用突变株UA13发酵产酶脱苦宜昌蜜橘果汁并进行理化指标检测,当酶解约50 min时,果汁中柚皮苷含量低于柚皮苷苦阈值;当酶解时间>75 min,果汁中仅剩下少量柚皮苷。使用高效液相色谱对脱苦结果验证检测,结果显示,突变株UA13产酶可有效水解果汁中的柚皮苷,且能提高果汁风味。因此,突变株UA13具有潜在的工业应用价值,为后续实验研究奠定基础。In order to screen out a high naringinase yielding mutant and explore its industrial application value,a naringinase producing strain Aspergillus tubingensis MN589840 was isolated from the mildewed pomelo peel.Then,a naringinase producing mutant UA13 was screened by the combination of UV irradiation and atmospheric and room temperature plasma(ARTP).The naringinase activity of UA13 was 41.524 U/mL,which was 3.06 times of that of MN589840.The results of naringinase hydrolysis conditions showed that the optimum temperature range was 40~50℃,and the optimum pH was 5.0.The naringinase produced by UA13 was used to debitter Yichang tangerine juice and the physical and chemical indexes of juice were detected.The results showed that the content of naringin in juice was lower than the threshold with the hydrolysis time of about 50 min,and only a small amount of naringin was left in the juice after 75 min of hydrolysis.High performance liquid chromatography(HPLC)was used to test the debittering results.The naringinase produced by UA13 could hydrolyze naringin effectively and improve the flavor of juice.Therefore,the mutant UA13 has industrial application potential,which lays a foundation for further research.

关 键 词：塔宾曲霉(Aspergillus tubingensis) 柚苷酶 常压室温等离子体 紫外诱变 复合诱变 脱苦 宜昌蜜橘 柚皮苷 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程]