红树林植物共附生放线菌的分离鉴定及其抗菌活性分析  

Isolation Identification and Antimicriobial Activity of Actinomycetes Associated with Mangrove Plants

作　　者：楚肖肖 周双清 许云[1] 吴文嫱 夏薇 张荣萍[2] 黄东益[2] 黄小龙[1] CHU Xiaoxiao;ZHOU Shuangqing;XU Yun;WU Wenqiang;XIA Wei;ZHANG Rongping;HUANG Dongyi;HUANG Xiaolong(Key Laboratory of Tropical Biological Resources of Ministry of Education,Haikou,Hainan 570228,China;College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China;College of Tropical Horticulture,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]热带生物资源教育部重点实验室,海口570228 [2]海南大学热带作物学院,海口570228 [3]海南大学热带园艺学院,海口570228

出　　处：《热带生物学报》2020年第4期406-414,共9页Journal of Tropical Biology

基　　金：2019年海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC133;2019RC069)。

摘　　要：笔者采用4种分离培养基对10份采集自海南岛西线海岸的红树林植物进行了茎的共附生放线菌分离,并利用16S rRNA序列分析法对分离得到的放线菌进行鉴定,采用滤纸片扩散法对分离得到的放线菌的发酵产物进行抗菌活性分析。结果共分离获得63株放线菌,初步确定16株代表性放线菌的分类地位。16株代表性放线菌中,有13株放线菌属于链霉菌属,有2株放线菌属于小单孢菌属,其中5株链霉菌为潜在的新物种。抗菌活性测试结果显示,16株代表性放线菌的发酵产物对至少1种指示菌具有抗菌活性。从菌株HNM0645的发酵粗提物中分离获得了1个化合物A,经过结构鉴定为已知化合物K-252a。通过测试不同浓度化合物A对山药炭疽病原菌的抑菌率,利用Excel求出毒力回归方程,计算出EC50值为286.61μg·mL−1,化合物A对供试细菌没有明显的抑菌活性。Ten samples containing actinomycetes were collected from mangrove plants in the west coast of Hainan Island and cultured on 4 isolation mediums for isolation.The isolated actinomycetes associated with the mangrove plant samples were identified by 16S rRNA sequence analysis,and the antimicrobial activities of fermented products of the isolated actinomycetes were analyzed by using filter paper diffusion method.The results showed that a total of 63 actinomycetes were isolated from the 10 mangrove plant samples.The taxonomic status of 16 representative strains of the isolated actinomycetes was established.Among the 16 representative strains 13 strains belong to the genus Streptomyces,of which 4 strains are of potential novel species,and 3 strains belong to the genus Micromonospora.The test of antimicrobial activity showed that the fermented products of 16 representative strains of the isolated actinomycetes have antimicrobial activity against at least one indicator bacterium or fungus.Compound A was isolated from the crude fermented extract of strain HNM0645,which was identified as known compound K-252a by structure.The inhibitory rates of the compound A against yam anthracnose pathogens(Colletotrichum gloeosporioides)were measured,and a toxicity regression equation was established by using Excel to calculate the EC50.The EC50 value of the compound A against the yam anthracnose pathogens was 286.61μg·mL−1,indicating the compound A had some antifungal activity against the yam anthracnose pathogen.The compound A was found to have no significant antibacterial activity against the tested bacteria.

关 键 词：红树林植物 放线菌 分离 鉴定 抗菌活性 

分 类 号：Q949.761.7[生物学—植物学] Q935