转录组和代谢组揭示独脚金内酯缺失影响水稻根和叶中的脂质和黄酮代谢  

Transcriptome and Metabolome Reveal that Loss of Strigolactone Affects Lipid and Flavonoid Metabolism in Rice Roots and Leaves

作　　者：张耀元 刘玲[1] 周秀娟 房传营 王守创 丁元昊 罗杰 ZHANG Yaoyuan;LIU Ling;ZHOU Xiujuan;FANG Chuanying;WANG Shouchuang;DING Yuanhao;LUO Jie(College of Tropical Crops,Hainan University,Haikou,Hainan 570228)

机构地区：[1]海南大学热带作物学院,海口570228

出　　处：《热带生物学报》2020年第4期415-425,共11页Journal of Tropical Biology

基　　金：海南省自然科学基金(319QN155);海南大学科研启动经费[KYQD(ZR)1916;KYQD(ZR)1866]。

摘　　要：对水稻的独脚金内酯合成突变体d17及野生型水稻ZH11进行转录组研究,结果表明,在d17的根和叶中的248和441个差异表达基因在脂质代谢路径上出现富集。代谢组检测结果表明,脂质和黄酮类物质的含量在d17突变体和ZH11中表现出显著差异。进一步研究发现,d17中的溶血磷脂和黄酮的含量显著降低,相关基因的表达水平下降,表明独脚金内酯参与调控水稻体内的黄酮和脂质代谢。研究结果可为研究独脚金内酯调控水稻体内次生代谢及生长发育提供参考。Transcriptome-based analysis of the strigolactone-deficient mutant d17 of rice(Oryza sativa L ssp.japonica)and its wild-type ZH11(O.sativa L.ssp.japonica cv.Zhonghua11)were performed.A total of 248 and 441 differentially expressed genes were found in the roots and leaves of d17,which were enriched on the lipid metabolism pathway.Metabolomics analysis revealed that lipid and flavonoid contents were significantly different between the d17 mutant and ZH11.Further research found that the contents of lysophospholipid and flavonoids in the d17 was decreased significantly,and that the expression levels of related genes were decreased.Our results revealed that strigolactone was involved in regulating of flavonoids and lipid metabolism in rice,which provides references for further research on strigolactone in regulating of secondary metabolism and growth in rice.

关 键 词：独脚金内酯 水稻 转录组 代谢组 黄酮 脂质 

分 类 号：S511[农业科学—作物学] Q946.885[生物学—植物学]