印迹基因PHLDA2在孕妇子痫前期胎盘中的差异表达和甲基化状态  

The expression and methylation statues of imprinting gene PHLDA2 in placenta of preeclampsia

作　　者：朱好 胡蓉 顾蔚蓉 ZHU Hao;HU Rong;GU Wei-rong(Department of Obstetrics,Obstetrics and Gynecology Hospital,Fudan University,Shanghai 200011,China)

机构地区：[1]复旦大学附属妇产科医院产科,上海200011

出　　处：《复旦学报（医学版）》2021年第1期128-131,共4页Fudan University Journal of Medical Sciences

基　　金：国家重点研发计划(2016YFC1000403)。

摘　　要：了解印迹基因PHLDA2在孕妇子痫前期胎盘中表达水平及甲基化状态变化,我们收集2017年6月—2018年12月于复旦大学附属妇产科医院分娩产妇的临床资料和胎盘组织,分为早发型子痫前期4例,晚发型子痫前期16例,正常对照组30例。采用Real-time PCR和焦磷酸甲基化测序检测和比较三组胎盘组织中印迹基因PHLDA2的相对表达量和甲基化情况。结果发现,与对照组比较,早发型子痫前期PHLDA2 mRNA的相对表达量差异无统计学意义(P=0.406),胎盘组织中PHLDA2启动子区甲基化程度差异性增加(P<0.001);而晚发型PHLDA2 mRNA的相对表达量有差异性下降(P=0.019),胎盘组织中PHLDA2启动子区甲基化程度差异性增加(P<0.001)。推测子痫前期孕妇胎盘组织中PHLDA2基因的低表达、启动子区高甲基化可能与子痫前期的发病有关。In order to investigate the expression level and methylation statues of imprinting gene PHLDA2 in placenta of preeclampsia,we collected the clinical information and samples of placenta tissues from women with early onset preeclampsia(n=4),late onset preeclampsia(n=20)and normal pregnancy(n=30)in Obstetrics and Gynecology of Fudan University during Jun 2017 to Dec 2018.Clinical characters were compared within the three groups.The m RNA expression of PHLDA2 was detected by real-time PCR and the methylation statues was detected by pyrosequencing.There was no significant difference in the relative m RNA expression level of PHLDA2 between early onset preeclampsia and normal group(P=0.405),while hypermethylation was found in promoter region of PHLDA2 in early onset preeclampsia group(P<0.001).The relative m RNA expression level of PHLDA2 was decreased in late onset preeclampsia group(P=0.019),while hypermethylation was found in promoter region of PHLDA2 in early onset preeclampsia(P<0.001).Low expression of PHLDA2 and hypermethylation of promoter region in placental tissues may be related to the pathogenesis of preeclampsia.

关 键 词：子痫前期 印迹基因 PHLDA2基因 胎盘 甲基化 

分 类 号：R714.245[医药卫生—妇产科学]