大麦基因组GRAS基因家族的全基因组鉴定与表达分析  

Genome-wide Identification and Phylogenetic Analysis of the GRAS Gene Family in Barley(Hordeum vulgare L.)

作　　者：孔豆豆 毛成志 王蕾[1,3] 王寒冬[1,3] 张怀刚[1,3] 徐金青[1,3] 沈裕虎 Kong Doudou;Mao Chengzhi;Wang Lei;Wang Handong;Zhang Huaigang;Xu Jinqing;Shen Yuhu(Key Laboratory of Adaptation and Evolution of Plateau Biota(AEPB),Qinghai Privincial Key Laboratory of Crop Molecular Breeding,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810001;College of Life Sciences,University of Chinese Academy of Sciences,Beijing,100049;Innovation Academy for Seed Design,Chinese Academy of Sciences(In Preparation),Xining,810001)

机构地区：[1]中国科学院西北高原生物研究所,中国科学院高原适应与进化重点实验室,青海省作物分子育种重点实验室,西宁810001 [2]中国科学院大学生命科学学院,北京100049 [3]中国科学院种子创新研究院(筹),西宁810001

出　　处：《分子植物育种》2021年第1期22-33,共12页Molecular Plant Breeding

基　　金：青海省科技成果转化专项(2019-NK-17);青海省创新平台建设专项项目(2017-ZJ-Y14);中科院科技服务网络(STS)计划区域重点项目“青海高寒农牧交错区饲草品种培育与推广”共同资助。

摘　　要：GRAS基因家族是一类仅存在于植物并广泛参与其生长发育调控的转录因子,根据其序列结构和系统发育树分化特征,GRAS转录因子包含PATI, DELLA, HAM, SCR, SHR等多个亚家族成员。本研究利用大麦最新的基因组数据库,采用生物信息学的方法筛选鉴定出41条GRAS基因序列,其中有34条序列具有完整的GRAS家族蛋白特有的GRAS结构域,可定位到大麦7条染色体上且呈不均匀分布。与拟南芥和水稻GRAS蛋白进行的系统发育分析,可将大麦GRAS家族蛋白进一步划分为10个亚家族。本研究对大麦GRAS基因的表达丰度分析,发现部分基因在发育阶段高表达,这可能暗示着这些基因在相应发育阶段起到重要作用。本研究结果可为后续挖掘和验证大麦GRAS基因提供参考。The GRAS gene family is a kind of transcription factor that exists only in plants and is widely involved in the regulation of its growth and development.According to its sequence structure and functional differentiation characteristics,GRAS transcription factors include PATI,DELLA,HAM,SCR,SHR and other subfamily members.In this study,we used the latest genomic database of barley to screen and identify 41 GRAS gene sequences by bioinformatics methods,among which 34 sequences have complete GRAS domain specific to GRAS family proteins.Among them,40 genes can be mapped to 7 chromosomes of barley and are unevenly distributed.Phylogenetic analysis with Arabidopsis and rice GRAS proteins further divides the barley GRAS family proteins into 10 subfamilies.Analysis of the abundance of barley GRAS gene revealed that some genes are highly expressed during development,which may imply that these genes play an important role in the corresponding developmental stages.This study can provide a reference for subsequent mining and verification of barley GRAS genes.

关 键 词：大麦(Hordeum vulgare L.) GRAS基因家族 系统发育分析 表达谱分析 

分 类 号：S512.3[农业科学—作物学]