藜麦DAPB基因丰度及生物信息学分析  

Gene Abundance and Bioinformatics Analysis of the DAPB Gene in Quinoa

作　　者：杨利艳[1] 杨雅舒 杨小兰 朱满喜 王创云[2] 邓妍[2] 赵丽[2] 郭虹霞[2] 张丽光[2] Yang Liyan;Yang Yashu;Yang Xiaolan;Zhu Manxi;Wang Chuangyun;Deng Yan;Zhao Li;Guo Hongxia;Zhang Liguang(School of Life Science,Shanxi Normal University,Linfen,041000;Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan,030031)

机构地区：[1]山西师范大学生命科学学院,临汾041000 [2]山西省农业科学院作物科学研究所,太原030031

出　　处：《分子植物育种》2021年第1期34-40,共7页Molecular Plant Breeding

基　　金：山西省应用基础研究计划项目(201901D111285);中国农业科学院科技创新工程协同任务(CAAS-XTCX20-190025-3);山西农谷建设科研专项项目(SXNGJSKYZX201704);山西省农业科学院博士后专项(YCX2018D2BH2)共同资助。

摘　　要：二氢砒啶二羧酸还原酶(DAPB)是赖氨酸合成途径中的一个重要酶,在蛋白质和细胞壁的合成中起着重要作用。本研究基于KEGG总数据库分析表明,藜麦中DAPB的基因拷贝数远高于水稻、玉米及大豆等作物。对藜麦基因组数据筛选得到4个DAPB基因成员,均属于碱性蛋白质,氨基酸序列长度约330 bp,分子量为36.623~36.880 k D,等电点(p I)为5.79~6.01,最大疏水系数为0.028~0.123,为疏水性蛋白,亚细胞定位于细胞质中,不存在跨膜区。4个DAPB基因之间的Ka/Ks值均小于0.5,表明该基因在进化过程中比较保守。系统进化分析发现,藜麦DAPB家族成员位于同一个亚家族,且与菠菜的亲缘关系较近。启动子顺式作用元件分析结果显示,DAPB家族除含有TATA-box和CAAT-box以外,还含有多个参与激素响应、光照、低温及其它应激反应相关的顺式作用元件和转录因子结合位点,暗示藜麦DAPB家族成员可能参与以上多种响应。本研究分析了藜麦DAPB基因的拷贝数并对DAPB进行了生物信息学分析,可为进一步了解DAPB基因家族的生物学功能提供参考。Dihydrodipicolinate reductase(DAPB)is an important enzyme in lysine synthesis,which also plays a vital role in protein synthesis and cell wall formation.Based on KEGG database analysis,we found the gene copy of DAPB in quinoa was much higher than that in rice,maize and soybean.To understand the function of DAPB gene,in the present study,four DAPB gene members were identified from the Chenopodium quinoa genome and their bioinformatics was analyzed.All the four DAPB are alkaline proteins,and amino acid sequences are about 330 bp.Molecular weights of DAPB are 36.623 k D to 36.880 k D,the theoretical isoelectric point(p I)ranges from 5.79 to6.01,and the maximum hydrophobic coefficient is between 0.028 and 0.123.The proteins locate in cytoplasm without transmembrane region presented.The Ka/Ks of the four DAPB genes are all less than 0.5,which indicate they were conserved during evolution process.Phylogenetic tree presented that the four DAPB genes were in the same subfamily,of which two of them were closely related to Spinacia oleracea L.DAPB promoter homeo-pathic elements analysis showed that besides TATA-box and CAAT-box,DAPB genes also contained multiple cis-acting elements and transcription factor binding sites that involved in hormone,light,low temperature and other st-ress responses,suggested that quinoa DAPB gene family might get involved in above factors response.The present study compared the gene copy of DAPB and conducted bioinformatics analysis of DAPB,which could provide information for further DAPB function study.

关 键 词：DAPB 藜麦(Chenopodium quinoa) 基因丰度 生物信息学 

分 类 号：S519[农业科学—作物学]