西南桦4CL基因的克隆及表达分析  

Cloning and Expression Analysis of 4CL Gene from Betula alnoides

作　　者：李云琴 原晓龙 王毅 张劲峰 Li Yunqin;Yuan Xiaolong;Wang Yi;Zhang Jinfeng(Yunnan Academy of Forestry and Grassland,Kunming,650201;The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration,The Key Laboratory of Forestry Plant Cultivation and Utilization,Yunnan Academy of Forestry and Grassland,Kunming,650201)

机构地区：[1]云南省林业和草原科学院,昆明650201 [2]云南省林业和草原科学院,云南省森林植物培育与开发利用重点实验室,国家林草局云南珍稀濒特森林植物保护和繁育重点实验室,昆明650201

出　　处：《分子植物育种》2021年第1期118-122,共5页Molecular Plant Breeding

基　　金：云南省林业科学院热带亚热带珍贵用材树种研发省创新团队(2017HC024);滇南西南桦高效培育技术研究(2016YFD0600604-02);国家自然科学基金项目(31860177)共同资助。

摘　　要：为研究西南桦(Betula alnoides)中木质素生物合成途径的关键酶4-香豆酸:辅酶A连接酶的调控机制,本研究采用RT-PCR技术从西南桦中克隆得到一个具有完整开放阅读框的4-香豆酸:辅酶A连接酶基因(Ba4CL, NCBI登录号为MK959316),该基因全长1 629 bp,共编码542个氨基酸。西南桦Ba4CL与光皮桦(Betula luminifera, ACJ38668.1) 4CL的蛋白序列同源性为99.63%,与白桦(Betula platyphylla, AAV65114.1)4CL同源性为99.45%;Ba4CL基因拥有4CL基因家族的保守域及具有保守序列SSGTTGLPKGV和GEICIRG,Ba4CL与桦木属植物白桦和光皮桦聚在一起。Ba4CL基因在西南桦的根、小枝、树皮和叶片中均有表达,且在小枝中表达量最高,树皮次之。本研究为培育西南桦优良用材品种提供参考。In order to study the metabolism mechanism of the key enzyme 4-coumarate:coenzyme A ligase in lignin biosynthesis pathway of Betula alnoides,used the RT-PCR technology,a complete open reading frame of Ba4 CL gene was cloned from B.alnoides for the first time.The full-length c DNA of Ba4 CL gene was 1629 bp,encoded 542 amino acids(NCBI accession number:MK959316).The protein sequence of Betula alnoides is 99.63%similar to the Betula luminifera(ACJ38668.1)and 99.45%Betula platyphylla(AAV65114.1),respectively.Sequence alignment analysis showed that amino acids had conserved amino acid blocks SSGTTGLPKGV and GEICIRG.The phylogenetic analysis revealed that Ba4 CL clustered together with B.platyphylla and B.Luminifera.Transcription pattern analysis showed that Ba4 CL expressed in different tissues and had the highest transcript profile in twig.The results provided theoretical references for the study of some important mechanisms in the formation of birch materials and cultivated the superior varieties of B.alnoides.

关 键 词：西南桦(Betula alnoides) 4-香豆酸:辅酶A连接酶 荧光定量PCR 基因功能分析 

分 类 号：S792.15[农业科学—林木遗传育种]