High multiple mutations of Plasmodium falciparum-resistant genotypes to sulphadoxine-pyrimethamine in Lagos, Nigeria  

作　　者：Hong Quan Uche Igbasi Wellington Oyibo Sunday Omilabu Shen-Bo Chen Hai-Mo Shen Chukwuma Okolie Jun-Hu Chen Xiao-Nong Zhou 

机构地区：[1]National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research,WHO Collaborating Center for Tropical Diseases,National Centre for International Research on Tropical Diseases,Ministry of Science and Technology,Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai,200025,People’s Republic of China [2]National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention-Shenzhen Center for Disease Control and Prevention Joint Laboratory for Imported Tropical Disease Control,Shanghai,200025,People’s Republic of China [3]Center for Infectious Diseases Research,Microbiology Department,Nigerian Institute of Medical Research,6 Edmund Crescent,Yaba,Lagos,Nigeria [4]ANDI Center of Excellence for Malaria Diagnosis,Department of Medical Microbiology and Parasitology,College of Medicine,University of Lagos,Lagos,Nigeria [5]Department of Medical Microbiology and Parasitology,College of Medicine,University of Lagos,Lagos,Nigeria [6]Department of Surveying and Geoinformatics,Faculty of Engineering,University of Lagos,Lagos,Nigeria

出　　处：《Infectious Diseases of Poverty》2020年第4期152-153,共2页贫困所致传染病（英文）

摘　　要：Background Plasmodium falciparum-resistance to sulphadoxine-pyrimethamine(SP)has been largely reported among pregnant women.However,the profile of resistance markers to SP dihydrofolate reductase(dhfr)and dihydropteroate synthase(dhps)in the general population are varied and not frequently monitored.Currently,SP is used as partner drug for artemisinin combination therapy(SP-artesunate)in some sub-Saharan African countries or as a prophylactic drug in intermittent preventive treatment of malaria during pregnancy and infants and in seasonal malaria chemoprevention(SMC).Profiling of P.falciparum-resistant genotypes to SP is dynamic and critical in providing data that would be useful for malaria control programmes.This study assessed the profile of dhfr and dhps genes genotypes among individuals with malaria in Lagos,Nigeria.Methods Molecular markers of SP resistance were identified by nested PCR and sequenced among malaria positive dried blood spots(DBS)that were collected from individuals attending health facilities from January 2013 to February 2014 and during community surveys from October 2010 to September 2011 across different Local Government Areas of Lagos State,Nigeria.Results A total of 242 and 167 samples were sequenced for dhfr and dhps,respectively.Sequence analysis of dhfr showed that 95.5%(231/242),96.3%(233/242)and 96.7%(234/242)of the samples had N51I,C59R and S108N mutant alleles,respectively.The prevalence of dhps mutation at codons A437G,A613S,S436A,A581G,I431V and K540E were 95.8%(160/167),41.9%(70/167),41.3%(69/167),31.1%(52/167),25.1%(42/167),and 1.2%(2/167)respectively.The prevalence of triple mutations(CIRNI)in dhfr was 93.8%and 44.3%for the single dhps haplotype mutation(SGKAA).Partial SP-resistance due to quadruple dhfr-dhps haplotype mutations(CIRNI-SGKAA)and octuple haplotype mutations(CIRNI-VAGKGS)with rate of 42.6%and 22.0%,respectively has been reported.Conclusions There was increased prevalence in dhfr triple haplotype mutations when compared with previous reports in the same environm

关 键 词：Plasmodium falciparum Antimalarial drug resistance Sulphadoxine-pyrimethamine Dihydrofolate reductase Dihydropteroate synthase 

分 类 号：R531.3[医药卫生—内科学]