Soluble IL-2Rα correlates with imbalances of Th1/Th2 and Tc1/Tc2 cells in patients with acute brucellosis  

作　　者：Hua-Li Sun Cheng-Jie Ma Xiu-Fang Du Si-Yuan Yang Xiao Lv Hong Zhao Ling-Hang Wang Yun-Xia Tang Xing-Wang Li Rong-Meng Jiang 

机构地区：[1]Department of Infectious Diseases,The Affiliated Hospital of Qingdao University,Qingdao,Shandong,China [2]The Laboratory of Infectious Diseases Centre,Beijing Di Tan Hospital,Capital Medical University,Beijing,China [3]Department of Infectious Diseases,the Third People Hospital,Linfen City,Shanxi Province,China [4]Department of Laboratory Medicine,the Third People Hospital,Linfen City,Shanxi Province,China [5]Center for Infectious Diseases,Beijing Di Tan Hospital,Capital Medical University,Beijing,China

出　　处：《Infectious Diseases of Poverty》2020年第4期170-170,共1页贫困所致传染病（英文）

摘　　要：Background Previous studies showed that soluble IL-2Rαis an important marker of cellular immune activation and might be a marker of treatment efficacy for children with brucellosis.However,data regarding adult patients with brucellosis were unknown.The aim of study was to explore the potential role of serum sIL-2Rαevaluating treatment responses in adult patients with brucellosis,and T cell immune status was also examined.Methods During January 2016–April 2017,30 patients with acute brucellosis from the Third People’s Hospital of Linfen in Shanxi Province and Beijing Di Tan Hospital,and 28 healthy controls were included in this study.Peripheral blood samples were collected before and after six weeks of antibiotic treatment.Serum sIL-2Rαlevels were measured by enzyme-linked immunosorbent assay,and the percentage of Th1,Th2,Tc1,Tc2,and Tregs was detected by flow cytometry after intracellular staining for cytokines(interferon-γand interleukin-4)and Foxp3 in T lymphocytes from peripheral blood.The obtained data were analyzed with Wilcoxon ranked sum tests for paired values,Mann-Whitney U-tests for comparisons between patients and healthy controls,and Spearman rank tests for correlation analyses.Results Serum sIL-2Rαlevels were significantly higher in patients than in controls(P=0.001).A significant decline was observed in patients after the cessation of treatment(P<0.001)and return to normal(P>0.05).Th1,Tc1,Th2,and Tc2 cell frequencies were higher in patients than in healthy subjects(P<0.05),while the Th1/Th2 and Tc1/Tc2 ratios were significantly lower(P=0.0305 and 0.0005,respectively)and returned to normal levels after treatment.In patients with acute brucellosis,serum sIL-2Rαlevels were negatively correlated with the Th1/Th2 ratio(r=−0.478,P=0.028),Tc1/Tc2 ratio(r=−0.677,P=0.001),and Tc1 percentage(r=−0.516,P=0.017).Serum sIL-2Rαand Tc2 percentages were positively correlated(r=0.442,P=0.045).Conclusions Based on the correlations with Th1/Th2 and Tc1/Tc2 ratios,serum sIL-2Rαlevels may reflect the im

关 键 词：BRUCELLOSIS SIL-2RΑ Tc1 Tc2 Th1 TH2 

分 类 号：R516.7[医药卫生—内科学]