甘蓝型油菜BnaLPAT2基因在拟南芥中的种子特异性表达及过表达分析  

Analysis of seed-specific expression and overexpression of BnaLPAT2 gene in Arabidopsis thaliana

作　　者：胡蓉 汪慧慧[1,2] 刘勇 熊兴华 HU Rong;WANG Huihui;LIU Yong;XIONG Xinghua(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Crop Gene Engineering Key Laboratory of Hunan Province,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]作物基因工程湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2021年第1期17-22,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：农业农村部转基因重大专项(SQ2018ZD080068);国家重点研发计划项目(2017YFD0101703);湖南省研究生科研创新项目(CX2018B428)。

摘　　要：以植物双元表达载体pBI121为骨架,将其改造成BnaLPAT2的过表达载体(p35S–BnaLPAT2)和种子特异性表达载体(pNapin–BnaLPAT2)。采用冻融法将重组质粒分别转入农杆菌感受态细胞(GV3101)中,通过农杆菌介导法转化拟南芥,分别获得阳性植株。对转基因植株种子的脂肪酸成分进行分析,结果表明:与野生型拟南芥相比,p35S–BnaLPAT2表达的后代植株种子油酸(C18∶1)相对含量降低了1.3,亚麻酸(C18∶3)相对含量提高了3.2,亚油酸(C18∶2)相对含量降低了0.7;pNapin–BnaLPAT2表达的后代植株种子油酸相对含量降低了1.29,亚麻酸相对含量提高了2.73,亚油酸相对含量提高了1.04,推测BnaLPAT2对亚麻酸的亲和力高于对亚油酸和油酸的亲和力。In this study,the plant binary expression vector pBI121 served as the backbone to transform into the BnaLPAT2 overexpression vector(p35S-BnaLPAT2)and the seed-specific expression vector(pNapin-BnaLPAT2)by use of freez-thaw transform method.Analysis of the fatty acids in the seeds of the transgenic plants showed that,compared to wild-type Arabidopsis thaliana,the relative content of seeds oleic acid of progeny plants expressing p35S-BnaLPAT2 decreased by 1.3 and the relative content of linolenic acid(C18∶3)increased by 3.2.The relative content of linoleic acid(C18∶2)was reduced by 0.7;the relative content of seeds oleic acid of progeny plants expressed by pNapin-BnaLPAT2 decreased by 1.29,the relative content of linolenic acid increased by 2.73,and the relative content of linoleic acid increased by 1.04.BnaLPAT2 has a higher affinity for linolenic acid than linoleic acid and oleic acid.

关 键 词：甘蓝型油菜 拟南芥 BnaLPAT2基因 过表达载体 脂肪酸 

分 类 号：S565.4[农业科学—作物学] Q786[生物学—分子生物学]