莪术醇的分离鉴定及体外抗肿瘤作用机制研究  

Isolation and Identification of Curcumol and Its Anti-Tumor Mechanism in Vitro

作　　者：罗球珠[1] 李穗晖[1] 黄楚燕 LUO Qiuzhu;LI Suihui;HUANG Chuyan(The First Affiliated Hospital of Guangehou University of Traditional Chinase Medicine,Guangehou,Guangdong,China 510407)

机构地区：[1]广州中医药大学第一附属医院,广东广州510407

出　　处：《中国药业》2021年第2期38-41,共4页China Pharmaceuticals

摘　　要：目的探讨莪术醇的分离鉴定方法及体外抗肿癌作用机制。方法莪术油减压蒸馏,收集重结晶得莪术醇,采用油醚-乙酸乙酯条带显色;体外培养宫颈癌细胞、肺癌细胞,采用四氮唑盐(MTT)法检测宫颈癌细胞、肺癌细胞的增殖情况;试验分为模型组、莪术醇组和莪术油组,采用流式细胞仪检测凋亡率,采用蛋白质印迹法检测肿瘤细胞Ki67和PCNA蛋白表达水平。结果减压蒸馏可分离得到莪术醇,其纯度为89.57%。莪术醇及莪术油均可有效抑制宫颈癌细胞、肺癌细胞的增殖,且莪术醇对宫颈癌细胞、肺癌细胞的抑制率高于莪术油。与模型组相比,莪术醇组和莪术油组细胞凋亡率、半胱氨酸蛋白酶3(Caspase-3)及B淋巴细胞瘤-2(Bcl-2)相关X蛋白(Bax)表达水平显著升高,Ki67,PCNA及Bcl-2蛋白表达水平显著降低(P<0.05);与莪术油组相比,莪术醇组细胞凋亡率、Caspase-3及Bax蛋白表达水平显著升高,Ki67,PCNA及Bcl-2蛋白表达水平显著降低(P<0.05)。结论莪术油通过减压蒸馏分离出的莪术醇,可有效抑制肿瘤细胞增殖并促进其凋亡,且莪术醇效果显著优于莪术油。Objective To investigate the isolation and identification of curcumol and its anti-tumor mechanism in vitro.Methods Zedoary turmeric oil was extracted by vacuum distillation and recrystallized to obtain curcumol,which was identified by oleyl ether-ethyl acetate strip.Cervical cancer cells and lung cancer cells were cultured in vitro,and their proliferation was detected by MTT.The cells were divided into model group,curcumol group and Zedoary turmeric oil group.The apoptosis rate was detected by flow cytometry,and the expression of Ki67 protein and PCNA protein in tumor cells was detected by Western blot.Results Curcumol could be isolated by vacuum distillation,and its purity was 89.57%.Both curcumol and Zedoary turmeric oil could effectively inhibit the proliferation of cervical cancer cells and lung cancer cells,and the inhibition rate of curcumol on cervical cancer cells and lung cancer cells is higher than that of Zedoary turmeric oil.Compared with those in the model group,the apoptosis rate and the expression levels of Caspase-3 and Bax protein in curcumol group and Zedoary turmeric oil group were significantly increased,while the expression levels of Ki67,PCNA and Bcl-2 protein in curcumol group and Zedoary turmeric oil group were significantly decreased(P<0.05).Compared with those in Zedoary turmeric oil group,the apoptosis rate and the expression levels of Caspase-3 and Bax protein in curcumol group were significantly increased,while the expression levels of Ki67,PCNA and Bcl-2 protein in curcumol group were significantly decreased(P<0.05).Conclusion Curcumol isolated from Zedoary turmeric oil by vacuum distillation can effectively inhibit the proliferation of tumor cells and promote their apoptosis,and the effect of curcumol is significantly better than that of Zedoary turmeric oil.

关 键 词：宫颈癌 莪术醇 莪术油 抗肿癌 细胞增殖 细胞凋亡 

分 类 号：R932[医药卫生—生药学] R284.2[医药卫生—药学]