乙醛脱氢酶2基因的可变剪接分析  

Alternative splicing analysis of the aldehyde dehydrogenase 2 gene

作　　者：夏万松 夏英 韦四喜 周春欢 杜洪 袁婷 金泳 黄海 XIA Wansong;XIA Ying;WEI Sixi;ZHOU Chunhuan;DU Hong;YUAN Ting;JIN Yong;HUANG Hai(School of Clinical Laboratory Science,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Clinical Laboratory,the First Hospital Attached to Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550001;Center of Clinical Laboratories,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Guiyang Hospital of Guizhou Aviation Industry Group,Guiyang 550009,Guizhou,China)

机构地区：[1]贵州医科大学医学检验学院,贵州贵阳550004 [2]贵州中医药大学第一附属医院检验科,贵州贵阳550001 [3]贵州医科大学附属医院临床检验中心,贵州贵阳550004 [4]贵航贵阳医院检验科,贵州贵阳550009

出　　处：《贵州医科大学学报》2021年第1期22-26,31,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81760429);贵州省科技创新人才团队项目(2019-5610);贵州省卫生计生委科学技术基金项目(gzwjkj2018-1-072);贵阳中医学院科研项目[2018(101)];贵阳市科技计划项目[筑科合同(2019)9-2]。

摘　　要：目的 探讨胃癌细胞中的乙醛脱氢酶2(ALDH2) m RNA发生可变剪接。方法 设计ALDH2基因特异性引物,用胃癌细胞(AGS、HGC-27、MKN45)、正常人胃黏膜上皮细胞GES-1和人外周全血白细胞提取RNA,进行逆转录聚合酶链反应(RT-PCR)扩增,然后对ALDH2扩增产物进行测序分析;使用DNAStar软件分析ALDH2变异体(V)的开放阅读框(ORF)。结果 ALDH2变异体1(V1)外显子2和外显子4发生可变剪接,V1外显子1的3’端和外显子3的5’端3个不同的位点发生可变剪接,V1存在8个长度超过100氨基酸的ORF。结论 ALDH2 m RNA存在多种可变剪接形式,提示其存在复杂的蛋白表达功能。Objective To investigate the occurrence of alternative splicing of aldehyde dehydrogenase2(ALDH2) mRNA in gastric cancer cells.Methods Specific primers for ALDH2 gene was designed,so as to extract the RNA from gastric cancer cells(AGS,HGC-27,MKN45),normal gastric mucosal epithelial cells(GES-1) and human peripheral blood leukocytes.Then he reverse transcriptase polymerase chain reaction(RT-PCR) was amplified,and the products of ALDH2 amplification were sequenced and analyzed;The open reading frame(ORF) of ALDH2 variant(V) was analyzed using DNAStar software.Results Alternative splicing site occurred in exon 2 and exon 4 of ALDH2 variant1(V1);Alternative splicing occurs at three different sites in the 3’end of V1 exon 1 and the 5’end of exon 3.Eight ORFs with more than 100 amino acids in length existed in V1.Conclusion In ALDH2 m RNA there exist multiple alternative splicing forms,suggesting that it has complex functions of protein expression.

关 键 词：胃肿瘤 胃肿瘤细胞 乙醛脱氢酶2 可变剪接 变异体 开放阅读框 

分 类 号：R392[医药卫生—免疫学]