耐旱根瘤菌对干旱胁迫下拉巴豆植株生长的影响  

Effects of drought-tolerance Rhizobium on the Dolichos lablab growth under drought stress

作　　者：张淑卿 臧丹丹 李剑峰 郭金梅 ZHANG Shuqing;ZANG Dandan;LI Jianfeng;GUO Jinmei(School of Geography and Resources, Guizhou Normal University, Guiyang, Guizhou 550018, China;Institute of Soil and Environment Bioremediation in Karst Habitats, Guizhou Normal University, Guiyang, Guizhou 550018, China)

机构地区：[1]贵州师范学院地理与资源学院,贵州贵阳550018 [2]贵州师范学院喀斯特生境土壤与环境生物修复研究所,贵州贵阳550018

出　　处：《干旱地区农业研究》2020年第6期39-45,共7页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金(31660691);贵州省科技计划项目(黔科合基础[2020]1Z077);贵州省科技奖励补助项目(黔科合平台人才[2018]5778-05);贵州省优秀青年科技人才培养对象专项资金项目(黔科合人字[2015]21号);贵州师范学院一流培育学科建设项目(2019YLPYXKB01);贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]264)资助。

摘　　要：为探明耐旱根瘤菌对干旱胁迫下拉巴豆(Dolichos lablab L.)植株生长的影响,以拉巴豆耐旱根瘤菌Rhizobium radiobacter Y-1(Y-1)、Rhizobium radiobacter 4-4(4-4)和Rhizobium radiobacter 37-1(37-1)作为供试菌株,润高拉巴豆种子经表面灭菌并催芽后接种菌液(以等量无菌水为对照),以砂-石屑基质(塑料盆上部为石英砂,下部为石漠岩屑)进行沙培盆栽实验。通过控制无菌水添加量来模拟重度石漠化生境下的轻度、中度及重度干旱胁迫,并测定各处理幼苗生长和生理相关指标。结果表明:干旱胁迫下接种耐旱根瘤菌Y-1和37-1能显著促进拉巴豆幼苗的生长,处理30 d后,菌液处理幼苗的生长和生理指标均优于对照;重度干旱胁迫(30%田间持水量(θf))下,Y-1菌液处理的株高、叶面积、根长、根鲜重及根体积较对照分别增加50.25%、73.17%、72.41%,136.00%和289.47%(P<0.05),原初光能转化效率Fv/Fm和可溶性蛋白含量分别提升42.09%和108.57%,而叶片MDA含量仅为对照的60.66%(P<0.05);轻度至中度干旱胁迫(45%~60%θf)下,37-1菌液处理的幼苗根长为对照的39.93%~47.99%,而MDA含量仅为对照的72.14%~90.92%。拉巴豆种子接种耐旱根瘤菌Y-1和37-1能促进幼苗株高、叶面积、根鲜重和根体积的增加,提升叶片可溶性蛋白含量及叶片原初光能转化效率,减轻叶片损伤并提高根系活力,表明接种耐旱根瘤菌可促进拉巴豆在干旱胁迫下的生长,提升幼苗的抗旱能力。To investigate the effects of drought-tolerant Rhizobium on the growth of Dolichos lablab seedlings under drought stress,a sand-culture experiment was conducted to evaluate the effect of Rhizobium radiobacter Y-1(Y-1),Rhizobium radiobacter 4-4(4-4),and Rhizobium radiobacter 37-1(37-1)on seedlings of Dolichos lablab under drought-stress condition.Surface sterilized and germinated seeds of Dolichos lablab cv.Rongai were sown in Plastics pots filled with clear sand at the top and rock debris(from rocky desertification area)at the bottom.The pots of each treatment were inoculated with sterilized water(control)or Rhizobium solution of Y-1,4-4,or 37-1.Calculated and supplied the sterilized water to simulate the mild,moderate,and severe drought stress in severe rocky desertification habitat.Results showed that the seedlings under drought stress were significantly promoted when seeds were inoculated with Rhizobium radiobacter Y-1 or Rhizobium radiobacter 37-1.On day 30,all of growth attributes and physiology of seedlings were significantly better than that of the control(inoculated with sterilized water)under drought stress.Under severe drought stress(30%of field water capacity,θf),the plant height,leaf area,root length,root fresh weight and root volume of seedlings inoculated with Y-1 increased by 50.25%,73.17%,72.41%,136.00%,and 289.47%,respectively,compared with those of the control.Plant soluble protein content increased by 108.57%and maximum quantum efficiency of PSⅡphotochemistry(Fv/Fm)increased by 42.09%compared with that of the control while leaf MDA content of Y-1 treatment was only 60.66%of the control(P<0.05).Under mild and moderate drought stress(45%~60%ofθf),plant root length of 37-1 treatment was 39.93%~47.99%of that of the control while MDA content of 37-1 treatment was only 72.14%~90.92%of that of the control.Inoculated with drought-tolerant Rhizobium radiobacter Y-1 and Rhizobium radiobacter 37-1 accelerated root growth,root volume,leaf area,and the formation of soluble protein,decreased leaf MDA con

关 键 词：耐旱根瘤菌 促生作用 拉巴豆 干旱胁迫 生理指标 石漠退化生境 

分 类 号：S541[农业科学—作物学] S154.381