干旱和盐胁迫对不同种源地麻花艽幼苗生长和生理特性的影响  

Effects of drought and salt stresses on growth and physiological characteristics of Gentiana straminea seedlings from different provenances

作　　者：程庭峰 王环[2] 陈世龙[1,3] 王久利 师生波 沈建伟[2] 周党卫 CHENG Tingfeng;WANG Huan;CHEN Shilong;WANG Jiuli;SHI Shengbo;SHEN Jianwei;ZHOU Dangwei(Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, ChineseAcademy of Sciences, Xining, Qinghai 810008, China;Analysis and Testing Center, Northwest Instituteof Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China;Key Laboratory of Crop MolecularBreeding of Qinghai Province, Xining, Qinghai 810008, China;College of Ecological Environment and Resources, QinghaiNationalities University, Xining, Qinghai 810007,China;University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院西北高原生物研究所高原生物适应与进化重点实验室,青海西宁810008 [2]中国科学院西北高原生物研究所分析测试中心,青海西宁810008 [3]青海省作物分子育种重点实验室,青海西宁810008 [4]青海民族大学生态环境与资源学院,青海西宁810007 [5]中国科学院大学,北京100049

出　　处：《干旱地区农业研究》2020年第6期46-51,共6页Agricultural Research in the Arid Areas

基　　金：青海省科技厅基础研究项目(2017-ZJ-702,2019-ZJ-7016);青海省自然基金项目(2019-ZJ-976Q);青海省创新平台建设专项项目(2017-ZJ-Y14)。

摘　　要：以青海省海北站、德令哈、河北乡、下藏科和班玛县5个地理种源麻花艽种子为材料,检测干旱胁迫、盐胁迫和混合胁迫下麻花艽幼苗的生长和生理特性。结果显示,胁迫下麻花艽材料的生长受到明显抑制,但不同地区受胁迫的影响不同,干旱胁迫下德令哈地区幼苗的株高最高,为2.36 cm;盐胁迫和混合胁迫下河北乡地区幼苗的株高最高,分别为2.49 cm和2.28 cm。不同胁迫下不同地区的幼苗生理特性也存在一定的差异,与对照相比,仅干旱胁迫下德令哈地区幼苗的MDA含量降低了69.07%,其可溶性蛋白增加了46.52%;单纯干旱胁迫下,海北站地区幼苗的POD和CAT活性分别增加了234.39%和86.39%;单纯盐胁迫处理下,海北站地区幼苗的POD和CAT活性分别增加了340.53%和127.86%;混合胁迫下德令哈地区幼苗的SOD和CAT分别增加了6.44%和51.20%。通过隶属函数法进行综合分析发现,5个地理种源麻花艽的抗旱性综合评价顺序为:德令哈>海北站>下藏科>河北乡>班玛县,耐盐性顺序为:海北站>下藏科>德令哈>班玛县>河北乡,混合胁迫抗性顺序为:德令哈>下藏科>河北乡>海北站>班玛县。结果表明,不同地理种源麻花艽对胁迫的响应存在一定的差异,德令哈和海北站地区麻花艽对胁迫的耐受性更强,推测可能与长期生长的自然环境有关。Seedlings of Gentiana straminea from five geographical provenances of Qinghai Province(Haibeizhan,Delingha,Hebeixiang,Xiazangke,and Banmaxian)were used as research materials to investigate growth and physiological characteristics of G.straminea under drought stress,salt stress,and mixed stress.The results showed that the growth of G.straminea was significantly inhibited under stress but impacts were different in different areas.The height of seedlings in Delingha area was the highest under drought stress,2.36 cm,and the highest in Hebeixiang area under salt stress and mixed stress,2.49 cm and 2.28 cm,respectively.The physiological characteristics also had certain differences.Only under drought stress,the MDA content of seedlings in Delingha area decreased by 69.07%,and the soluble protein increased by 46.52%;under simple drought stress,the POD and CAT activities of seedlings in Haibeizhan area increased by 234.39%and 86.39%,respectively;under the simple salt stress treatment,the POD and CAT activities of seedlings in Haibeizhan increased by 340.53%and 127.86%,respectively.The SOD and CAT of seedlings in Delingha area also increased by 6.44%and 51.20%,respectively,under mixed stress.From a comprehensive evaluation using membership function method,the drought resistance of G.straminea ranked as follow:Delingha>Haibeizhan>Xiazangke>Hebeixiang>Banmaxian;salt tolerance:Haibeizhan>Xiazangke>Delingha>Banmaxian>Hebeixiang;mixed stress resistance:Delingha>Xiazangke>Haibeizhan>Haibeixiang>Banmaxian.The results showed that different geographical provenances of G.straminea had some differences in response to stress,and G.straminea in Delingha and Haibeizhan areas were more tolerant to stress,presumably related to the long-term growth of the natural environment.

关 键 词：麻花艽 干旱胁迫 盐胁迫 地理种源 生理特性 

分 类 号：S567.239[农业科学—中草药栽培] Q945.78[农业科学—作物学]