金铁锁转录组WRKY转录因子基因的克隆及分析  

Cloning and Analysis of WRKY Transcription Factor Gene in Psammosilene tunicoides Transcriptome

作　　者：江舟 钱子刚 张爱丽 JIANG Zhou;QIAN Zi-gang;ZHANG Ai-li(Department of Basic Medicine,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;Department of TCM,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学基础医学院,云南昆明650500 [2]云南中医药大学中药学院,云南昆明650500

出　　处：《现代中药研究与实践》2020年第6期15-19,共5页Research and Practice on Chinese Medicines

基　　金：云南省科学技术厅-云南中医学院应用基础研究联合专项(2017FF117-033)。

摘　　要：目的克隆金铁锁PtWRKY转录因子全长基因,分析其蛋白序列特征并预测转录因子潜在的结合区域信息。方法克隆两个全长基因PtWRKY1和PtWRKY2,并使用生物信息学方法对两条转录因子的理化性质及同源性进行分析。结果克隆得到的两条转录因子所编码的蛋白为分泌蛋白;两条转录因子的亚细胞定位于细胞核,均具有WRKYGQK保守结构域;PtWRKY1和PtWRKY2与藜麦的WRKY转录因子有较高的同源性。结论为进一步研究PtWRKY转录因子对金铁锁三萜皂苷次生代谢合成的调控机制提供基础。Objective To clone the full-length gene of PtWRKY transcription factor,analyze and predict its protein sequence characteristics and potential transcription factor binding site information.Methods Two full-length genes PtWRKY1 and PtWRKY2 are cloned,and the physicochemical properties and homology of the two transcription factors are analyzed by bioinformatics methods.Results The proteins encoded by the two transcription factors are secretory proteins.The subcells of the two transcription factors are located in the nucleus and both have WRKYGQK conserved domain.PtWRKY1 and PtWRKY2 have high homology with WRKY transcription factors of quinoa.Conclusion It will provide a basis for further research on the regulation mechanism of PtWRKY transcription factor on the secondary metabolic synthesis of triterpenoid saponins.

关 键 词：金铁锁 转录因子 WRKY 蛋白序列分析 

分 类 号：R282.5[医药卫生—中药学]