基于抵当汤减毒的钩吻生物碱体内外含量变化研究  

Study on Changes of Chemical Composition of Alkaloids of Gelsemium elegans Based on Didang Decoction after Reducing Toxicity

作　　者：王文义 檀兴慧 王婉莹 谌赛男 廖华军[1] 吴水生[1] 李德森[1] WANG Wenyi;TAN Xinghui;WANG Wanying;CHEN Sainan;LIAO Huajun;WU Shuisheng;LI Desen(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中国中医药信息杂志》2021年第1期100-106,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：福建省自然科学基金(2019J01342);福建中医药大学科研平台校管课题(X2019008-平台)。

摘　　要：目的建立体内、体外钩吻生物碱成分测定方法,并测定抵当汤减毒前后钩吻生物碱成分含量,探讨抵当汤减缓钩吻毒性的可能作用机制。方法采用UPLC-QDa体外检测钩吻与抵当汤混合前后6种生物碱成分变化,采用UPLC-MS/MS检测抵当汤联合钩吻灌胃对小鼠血浆中钩吻生物碱成分含量的影响。结果与抵当汤混合后,钩吻中钩吻素子、钩吻素甲、钩吻绿碱、胡蔓藤碱乙含量明显降低(P<0.01);抵当汤+钩吻组小鼠血清钩吻素子、钩吻素甲、钩吻素己、钩吻绿碱、胡蔓藤碱乙、胡蔓藤碱丙含量均较钩吻组明显降低(P<0.01)。结论降低钩吻素子、钩吻素甲、钩吻素己、钩吻绿碱及胡蔓藤碱乙含量可能是抵当汤减缓钩吻毒性的作用机制之一。Objective To establish a method for determination of alkaloids of Gelsemium elegans in vitro and in vivo;and to study the content of alkaloid components of Gelsemium elegans before and after reducing toxicity;To explore the possible mechanism of the action of Didang Decoction in the process of reducing toxicity.Methods UPLC-QDa method was used to detect the changes of 6 alkaloids after combined use of Gelsemium elegans and Didang Decoction in vitro.UPLC-MS/MS method was established to detect the changes of plasma alkaloids in mice fed with Gelsemium elegans combined with Didang Decoction.Results The contents of koumine,gelsemine,gelsevirine and humantenine of Gelsemium elegans were signifantly decreased under the action of Didang Decoction(P<0.01).The contents of koumine,gelsemine,gelsenicine,gelsevirine,humantenine and humantenidine in group of Didang Decoction+Gelsemium elegans were significantly decreased compared with Gelsemium elegans group(P<0.01).Conclusion The decrease of koumine,gelsemine,gelsenicine,gelsevirine and humantenine of Gelsemium elegans may be one of the mechanisms of Didang Decoction to reduce the toxicity of Gelsemium elegans.

关 键 词：抵当汤 钩吻总碱 UPLC-QDa UPLC-MS/MS 含量变化 

分 类 号：R285.5[医药卫生—中药学]