三叶青黄酮对荷Lewis肺癌小鼠免疫功能及肿瘤组织凋亡相关蛋白的影响  

Effects of Tetrastigma hemsleyanum Diels et Gilg flavone on immune function and apoptosis-related proteins in tumor tissues of Lewis lung cancer mice

作　　者：林钰久 柴树人 龙坤兰 陈骏 石冀敏 徐静静[1] LIN Yu-jiu;CHAI Shu-ren;LONG Kun-lan;CHEN Jun;SHI Ji-min;XU Jing-jing(University of Electronic Science and Technology Hospital,Chengdu 610054,China;Ningbo Hospital of Traditional Chinese Medicine,Ningbo 315000,China)

机构地区：[1]电子科技大学医院,成都610054 [2]浙江省宁波市中医院,宁波315000<>

出　　处：《天然产物研究与开发》2021年第1期8-15,共8页Natural Product Research and Development

基　　金：国家中医药管理局中医药标准化(SATCM-2015-BZ(200))。

摘　　要：探索三叶青黄酮对荷Lewis肺癌小鼠免疫功能及肿瘤组织凋亡相关蛋白的影响。将60只小鼠随机分为对照组、模型组、三叶青黄酮高、中、低剂量组(100、50、25 mg/mL)各10只。连续干预14天后,检测各组胸腺指数、脾脏指数、移植瘤组织凋亡及凋亡相关蛋白表达,检测外周血调节性T细胞(Treg)比例。结果显示,与模型组比,三叶青黄酮高中剂量组胸腺指数、脾脏指数和移植瘤组织凋亡率升高(P<0.05),凋亡相关蛋白表达升高(P<0.05),外周血CD4+CD25+Foxp3+Treg细胞所占比例降低(P<0.05)。综上,三叶青黄酮可降低荷Lewis肺癌小鼠Treg细胞比例,提高免疫功能,诱导移植瘤组织凋亡。To explore effects of Tetrastigma hemsleyanum Diels et Gilg flavone(THDEGF)on immune function and apoptosis-related proteins in tumor tissues of Lewis lung cancer mice.Sixty mice were randomly divided into control group,model group,high-dose,medial-dose and low-dose THDEGF group(100,50 and 25 mg/mL),10 cases in each group.They were continuously intervened for 14 days.After intervention,thymus index and spleen index in each group were detected.The apoptosis of transplanted tumor tissue was observed by HE staining and TUNEL staining.The expression of apoptosis-related protein was detected by Western blot.The ratio of regulatory T cells(Treg)in peripheral blood were detected by flow cytometer.The result showed that compared with model group,thymus index,spleen index and apoptosis rate of transplanted tumor tissues were significantly increased in high-dose THDEGF group and medial-dose THDEGF group(P<0.05),while expression of B-cell lymphoma/leukemia-2(Bcl-2)was decreased(P<0.05),expression of Bcl2-associated X protein(Bax)and cleaved cysteine aspartase 3(cleaved Caspase-3)were increased(P<0.05),proportion of CD4+CD25+Foxp3+Treg cells was significantly decreased(P<0.05).With the increased dose of THDEGF,its effects were significantly enhanced(P<0.05).In conclusion,THDEGF can reduce the proportion of Treg cells in Lewis lung cancer mice,improve the cellular immune function,and induce the apoptosis of transplanted tumor tissues.

关 键 词：三叶青黄酮 肺癌 免疫功能 凋亡 

分 类 号：R331[医药卫生—人体生理学]