十二酰基-β-环糊精聚砜共混膜的制备及其在色氨酸手性拆分中的应用  

Preparation of Dodecyl-β-cyclodextrin Polysulfone Blend Membrane and Its Application in Chiral Resolution of Tryptophan

作　　者：白小平 罗欢 陈建秋 季一兵 BAI Xiaoping;LUO Huan;CHEN Jianqiu;JI Yibing(Key Lab.of Drug Quality Control and Pharmacovigilance,Ministry of Education,School of Science,China Pharmaceutical University,Nanjing 210009)

机构地区：[1]中国药科大学理学院,教育部药物质量与安全预警重点实验室,江苏南京210009

出　　处：《中国医药工业杂志》2021年第1期112-119,共8页Chinese Journal of Pharmaceuticals

基　　金：江苏省自然科学基金(BE2019717)。

摘　　要：本研究以十二酰基-β-环糊精为手性选择剂,采用浸入沉淀相转化法制备十二酰基-β-环糊精聚砜共混膜,对膜的稳定性和性能进行考察,并将其用于色氨酸外消旋体的手性拆分。同时建立能够拆分色氨酸外消旋体的手性配体交换液相色谱法,并进行方法学考察,用于评价共混膜的手性拆分能力。采用BaseLine C18柱(4.6 mm×250 mm,5μm),流动相为水相(0.375 mmol/L L-苯丙氨酸和0.075 mmol/L硫酸铜溶液,冰醋酸调至pH 4.3)∶甲醇(80∶20),检测波长为278 nm,柱温为35℃,流速为1 ml/min。建立的方法为手性拆分色氨酸对映体提供了参考。In this study,dodecanoyl-β-cyclodextrin was used as a chiral selector to prepare a dodecanoyl-β-cyclodextrin polysulfone blend membrane by immersion precipitation phase inversion method.The stability and performance of the membrane were investigated,and the membrane was used for the chiral resolution of tryptophan racemates.In addition,a chiral ligand exchange liquid chromatography method,which was capable of resolving tryptophan racemates,was established to evaluate the chiral resolution of the blended membrane,and the methodological investigation was conducted.A BaseLine C18 column(4.6 mm×250 mm,5μm)was used,with the mobile phase of aqueous phase(0.375 mmol/L L-phenylalanine and 0.075 mmol/L copper sulfate solution,adjusted to pH 4.3 with glacial acetic acid)-methanol(80∶20).The detection wavelength was 278 nm,the column temperature was 35℃,and the flow rate was 1 ml/min.The established method provided a reference for chiral resolution of tryptophan enantiomers.

关 键 词：色氨酸 手性配体交换 手性拆分 共混膜 

分 类 号：O652.6[理学—分析化学]