新疆牛羊产业链中金黄色葡萄球菌的污染调查与毒力基因检测  

Investigation of Staphylococcus aureus Pollution and Virulence Gene Detection in Xinjiang Cattle and Sheep Industry Chain

作　　者：胥兰 刘英玉[1] 麦多 朱明月 蒋金豆 卢炜 朱梦含 郑晓风[1] 彭斌[1] XU Lan;LIU Yingyu;MAI Duo;ZHU Mingyue;JIANG Jindou;LU Wei;ZHU Menghan;ZHENG Xiaofeng;PENG Bin(College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2021年第1期182-189,共8页Xinjiang Agricultural Sciences

基　　金：国家重点研发计划“牛羊养殖、屠宰和牛奶生产过程中重要食源性病原微生物的流行与传播规律研究”(2018YFD0500500);2017年优秀青年科技人才项目“新疆地区致病性STEC菌株作用于HIEC细胞的机制研究”(2017Q021);2020年度校级大学生创新项目(188)。

摘　　要：【目的】分析乌鲁木齐及周边地区牛羊源金黄色葡萄球菌及耐甲氧西林金黄色葡萄球菌的污染状况及常见毒力基因检测。【方法】采集乌鲁木齐及周边地区牛养殖场和牛羊屠宰场752份样品(粪样、挤奶厅工具拭子、胴体拭子、屠宰工具拭子等),采用国家标准方法进行金黄色葡萄球菌分离和耐甲氧西林金黄色葡萄球菌的鉴定,检测10种常见毒力基因(sea、seb、sec、fnbA、fnbB、hla、hlb、clfa、pvl、tst)的编码情况。【结果】共分离出26株金黄色葡萄球菌,分离率为3.46%,其中奶牛养殖场、牛屠宰场和羊屠宰场的分离率分别是4.39%、1.13%、3.72%、。其中有8株是耐甲氧西林金黄色葡萄球菌,占检出率的30.77%。毒力基因sea、seb、sec、fnbA、fnbB、hla、hlb、clfa、pvl、tst的检测率分别是11.54%、19.23%、3.85%、3.85%、23.08%、100%、53.85%、100%、0、15.38%,其中clfa和hla的检出率最高。【结论】新疆奶牛养殖场和牛羊屠宰场中均存在金黄色葡萄球菌,其中奶牛养殖场中MRSA的污染较严重,金黄色葡萄球菌主要编码hla和clfa 2种毒力基因。【Objective】Staphylococcus aureus is one of the more common pathogenic bacteria in nature.Methicillin-resistant Staphylococcus aureus is epidemic and spread worldwide,and has quickly become an important pathogen in hospital and community-acquired infections.The purpose of this study is to understand the contamination status of Staphylococcus aureus and methicillin-resistant staphylococcus aureus from cattle and sheep in Urumqi and surrounding areas and to detect common virulence genes.【Methods】752 samples from cattle farms and cattle and sheep slaughterhouses in Urumqi and surrounding areas(faecal samples,milking parlor tool swabs,carcass swabs,slaughter tool swabs,etc.)were collected and Staphylococcus aureus was performed using national standard methods:isolation and identification of methicillin-resistant Staphylococcus aureus,and 10 common virulence genes detected were:sea,seb,sec,fnbA,fnbB,hla,hlb,clfa,pvl,tst.【Result】A total of 26 strains of Staphylococcus aureus were isolated with an isolation rate of 3.46%.Among them,the isolation rates of dairy farms,cattle slaughterhouses and sheep slaughterhouses were 4.39%,1.13%and 3.72%,respectively.Of these,8 strains were methicillin-resistant Staphylococcus aureus,accounting for 30.77%of the detection rate.The detection rates of virulence genessea,seb,sec,fnbA,fnbB,hla,hlb,clfa,pvl,and tst were 11.54%,19.23%,3.85%,3.85%,23.08%,100%,53.85%,100%,0,15.38%,with the highest detection rate of clfa and hla.。【Conclusion】Staphylococcus aureus is present in Xinjiang dairy farms and cattle and sheep slaughterhouses.Among them,MRSA pollution is serious in dairy farms.Staphylococcus aureus mainly encodes two virulence genes,hla and clfa.

关 键 词：新疆 牛羊 金黄色葡萄球菌 毒力基因 

分 类 号：S851.3[农业科学—预防兽医学] S826[农业科学—兽医学]