棉铃虫唾液凝集素的初步研究  

A preliminary study of lectin in the saliva(regurgitant)of Helicoverpa armigera

作　　者：王雄雅[1,2] 李星 白素芬[1] 李欣[1] WANG Xiongya;LI Xing;BAI Sufen;LI Xin(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]中国农业科学院植物保护研究所,北京100193

出　　处：《植物保护》2021年第1期91-96,共6页Plant Protection

基　　金：河南省自然科学基金(182300410089);河南省高等学校重点科研项目(17A210017)。

摘　　要：昆虫唾液在成功取食寄主中发挥关键作用。而有关植食性昆虫唾液(反吐液)的研究多集中在与寄主植物的互作关系上,鲜有涉及唾液免疫因子的研究。凝集素作为模式识别受体,在病原物的识别中发挥重要作用,是昆虫重要的免疫因子。棉铃虫幼虫血淋巴中已报道的凝集素种类至少有8种,但其唾液中是否存在凝集素却未见报道。本文通过与鸡血红细胞的血凝反应检测到棉铃虫幼虫唾液中含有凝集活性物质,凝集效价高达29。显微观察显示,唾液对鸡血红细胞具有明显的凝集作用。经35%和45%硫酸铵盐析沉淀获得的组分具有明显凝集活性,表明唾液中的凝集活性物质为蛋白质。通过糖抑制试验,发现棉铃虫唾液凝集素与其血淋巴凝集素的糖结合谱存在差异,表明他们是不同的凝集素种类。棉铃虫唾液凝集素的揭示为理解植食性昆虫唾液的免疫功能提供了新的证据。Insect saliva plays a major role in successful feeding on host.In recent years,the researches on salivary components of herbivory insects have mainly focused on the co-evolution and relationships between insects and plants,and less attention have been paid to the factors involved in immunity.Lectins,as pattern recognition receptors,play important roles in recognizing foreign substances in the insect immune system.There are at least eight C-type lectins in larval hemolymph of Helicoverpa armigera,but until now no lectin has ever been reported in its saliva(regurgitant).In the present study,the agglutination activity of H.armigera saliva was detected by hemagglutination reaction with chicken erythrocytes.The agglutination reaction of chicken erythrocytes treated with H.armigera saliva was observed obviously under phase contrast microscope.By ammonium sulfate precipitation,the protein precipitated by 35%and 45%ammonium sulfate showed obvious agglutination activity,indicating that the substance with agglutination activity in saliva was a protein.The carbohydrate-inhibition assay discovered the difference between saliva and hemolymph in carbohydrate-binding profiles of lectin,suggesting that they were different lectins.The lectin identified in saliva of H.armigera adds new proof to the understanding of immunity of phytophagous insects.

关 键 词：凝集素 棉铃虫 唾液(反吐液) 凝集活性 糖抑制试验 

分 类 号：Q965[生物学—昆虫学]