良恶性肺结节CD31表达水平的影像空间异质性差异  

A comparative study of CD31 expression level and image spatial heterogeneity between benign and malignant pulmonary nodules

作　　者：洪俊杰 刘博乐 陈瑶[2] 唐坤[1] 薛蓓慧 吴孙婕 郑祥武[1] HONG Junjie;LIU Bole;CHEN Yao;TANG Kun;XUE Beihui;WU Sunjie;ZHENG Xiangwu(Department of Radiology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Department of Pathology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China)

机构地区：[1]温州医科大学附属第一医院放射科,浙江温州325015 [2]温州医科大学附属第一医院病理科,浙江温州325015

出　　处：《温州医科大学学报》2021年第2期142-145,共4页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(LY17H180009)。

摘　　要：目的:探讨CD31表达水平相关的微血管密度(MVD)的空间异质性特征在良恶性肺结节中的差异。方法:收集术后肺结节的组织标本35例,其中鳞癌7例,腺癌23例,炎性结节5例。采用免疫组化法检测肺结节近心端与远心端的微血管标志物CD31的阳性表达水平。以配对t检验及独立样本t检验分析良恶性结节近心端与远心端的CD31组内及组间的差异。结果:30例恶性结节近远心端的CD31表达分别为19.2±8.6和13.3±6.8,差异有统计学意义(P<0.05)。5例良性结节近远心端的CD31表达分别为28.2±10.0和26.8±3.0,差异无统计学意义(P>0.05)。良恶性组间对比分析:良性结节远心端的CD31表达较恶性结节高,差异有统计学意义(P<0.05);良恶性结节近心端间的CD31表达差异无统计学意义(P>0.05)。结论:良恶性肺结节近远心端CD31表达相关的MVD存有差异,可为影像学良恶性肺结节的空间异质性差异特征提供病理学依据。Objective:To analyze the spatial heterogeneity of microvascular density(MVD)in benign and malignant pulmonary nodules based on CD31 expression level.Methods:Thirty-five cases of paraffin tissue specimens were collected after wedges resection of pulmonary nodules,including 7 cases of squamous cell carcinoma,23 cases of adenocarcinoma and 5 cases of inflammatory nodules.Immunohistochemistry was used to detect the expression level of CD31,a microvascular marker in the proximal and distal pulmonary nodules.Paired t-test and independent sample t-test were used to analyze the difference of CD31 expression level between intragroup and inter-group.Results:CD31 expression in the proximal/distal part of the 30 malignant nodules was 19.2±8.6 and 13.3±6.8,respectively,with significant difference(P<0.05).The CD31 expression in the proximal/distal part of 5 benign nodules was 28.2±10.0 and 26.8±3.0,respectively,without statistical difference(P>0.05).The comparison between benign and malignant groups showed that the expression of CD31 at the distal part of benign nodules was higher than that of malignant nodules(P<0.05).No significant difference was found in the expression of CD31 at the proximal part of benign and malignant nodules(P>0.05).Conclusion:Based on CD31 expression level,difference exists in MVD of proximal/distal part between benign and malignant pulmonary nodules,which provides pathological basis for the spatial heterogeneity of benign/malignant pulmonary nodules.

关 键 词：CD31 肺癌 免疫组织化学 微血管密度 

分 类 号：R445[医药卫生—影像医学与核医学]