多参数预测人巨细胞病毒临床病毒株UL145基因B细胞表位  

Multi-parameter prediction of B cell epitope of UL145 gene of human cytomegalovirus clinical strain

作　　者：伍苑宾[1] 胡兢晶[1] 王波[1] 苏海浩[1] 谭琪琪 WU Yuanbin;HU Jingjing;WANG Bo;SU Haihao;TAN Qiqi(Pediatrics Department,Guangdong Maternal and Child Health Hospital,Guangzhou511442,China)

机构地区：[1]广东省妇幼保健院儿科,广东广州511442

出　　处：《中国现代医生》2020年第35期27-30,共4页China Modern Doctor

基　　金：广东省自然科学基金资助项目(2016A030313788)。

摘　　要：目的应用多参数预测及分析人巨细胞病毒(Human cytomegalovirus,HCMV)临床病毒株UL145基因B细胞表位。方法根据人巨细胞病毒UL145基因氨基酸序列预测其二级结构,结合跨膜结构、亲水性、可及性及抗原性方案等参数综合预测UL145基因B细胞表位。结果HCMV pUL145氨基酸等电点为6.64,二级结构以无规则卷为主,1~130位氨基酸均为膜外区域,结合多种预测方法分析提示,88~99位氨基酸序列内可作为HCMV UL145基因的B细胞候选表位。结论多参数预测提示88~99位氨基酸序列为HCMV UL145基因B细胞表位预测序列,为进一步制备相应的抗体提供了重要依据。Objective To predict and analyze B cell epitope of UL145 gene of human cytomegalovirus(HCMV)clinical strain by using multi-parameter method.Methods According to the amino acid sequence of HCMV UL145 gene,the secondary structure was predicted.Combined with transmembrane structure,hydrophilicity,accessibility,antigenicity scheme and other parameters,the B cell epitope of UL145 gene was predicted comprehensively.Results The amino acid isoelectric point of HCMV pUL145 was 6.64,the secondary structure was dominated by random curling,and amino acids 1-130 were all extra-membranous regions.Combined with various prediction methods,it was suggested that amino acid sequences at positions 88-99 could be used as B cell candidate epitopes of HCMV UL145 gene.Conclusion Multi-parameter prediction indicates that the amino acid sequences at positions 88-99 are predicted sequences of B cell epitopes of HCMV UL145 gene,which provide an important basis for further preparation of corresponding antibodies.

关 键 词：人巨细胞病毒 UL145 B细胞表位 生物信息学分析 

分 类 号：R39[医药卫生—基础医学] R71