秦皮甲素对高糖诱导大鼠肾小球系膜细胞增殖及纤维连接蛋白表达的影响  

Roles of Esculin in Cell Proliferation and Fibronectin Protein Expression Level in Rat Glomerular Mesangial Cells Exposed to High Glucose Condition

作　　者：包昀 林双峰[3] 邓波 刘彬[1] 刘少军[1] 黎佼[1] 庄佳纯 李雨卓 刘慰华[1] BAO Yun;LIN Shuangfeng;DENG Bo;LIU Bin;LIU Shaojun;LI Jiao;ZHUANG Jiachun;LI Yuzhuo;LIU Weihua(The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou Institute of Cardiovascular Disease,Guangzhou 510260 Guangdong,China;School of Pharmaceutical Sciences,Guangzhou Medical University,Guangzhou 511436 Guangdong,China;The first Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China)

机构地区：[1]广州医科大学附属第二医院广州心血管疾病研究所,广东广州510260 [2]广州医科大学药学院,广东广州511436 [3]广州中医药大学第一附属医院,广东广州510405

出　　处：《中药新药与临床药理》2021年第2期214-218,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金面上项目(广东省基础与应用基础研究基金,2019A1515011214);广州市属高校产学研结合基地项目(14CXY03);广东省教育厅特色创新项目(2016KTSCX113);广州医科大学2019年度大学生实验室开放项目(广医大发[2019]82号)。

摘　　要：目的探讨秦皮甲素对高糖诱导的大鼠肾小球系膜细胞(HBZY-1)增殖及纤维连接蛋白(FN)表达的影响。方法采用CCK-8法检测不同浓度秦皮甲素(10、30、90μmol·L-1)对正常培养条件(含糖5.6 mmol·L-1)和高糖培养条件(含糖25 mmol·L-1)下的HBZY-1细胞增殖的影响;以PI3K抑制剂Wortmannin(1μmol·L-1)干预为对照,采用Western Blot法检测秦皮甲素对高糖培养条件下HBZY-1细胞FN蛋白表达及PI3K/Akt蛋白磷酸化水平变化的影响。结果在正常培养条件下,与正常组比较,秦皮甲素给药组(10、30、90μmol·L-1)的HBZY-1细胞增殖均无明显变化(P>0.05)。在高糖培养条件下,与正常组比较,高糖组的细胞活力明显增加(P<0.05),HBZY-1细胞FN蛋白表达和PI3K/Akt磷酸化水平明显升高(P<0.05);与高糖组比较,90μmol·L-1秦皮甲素给药组的HBZY-1细胞活力明显降低(P<0.05),FN蛋白表达和PI3K/Akt磷酸化水平亦显著下调(P<0.05)。结论秦皮甲素能够抑制高糖诱导的HBZY-1细胞增殖,下调FN蛋白表达,可能与其抑制PI3K/Akt信号通路激活密切相关。Objective To observe the roles of Esculin in cell proliferation and fibronectin(FN)protein expression of rat glomerular mesangial cells(HBZY-1)induced by high glucose.Methods The CCK-8 method was used to detect the effect of different concentrations of Esculin(10,30,90μmol·L-1)on the proliferation of rat glomerular mesangial cells under normal culture condition(5.6 mmol·L-1 glucose)and high glucose condition(25 mmol·L-1glucose).PI3 K inhibitor Woitmannin(1μmol·L-1)treatment served as control.Western Blot analysis was used to detect protein expression of FN and the phosphorylation of PI3 K/Akt in glomerular mesangial cells.Results Under normal culture condition,the mesangial cell proliferation did not change significantly in the Esculin group(10,30,90μmol·L-1)compared with the normal group(P>0.05).Under high glucose condition,compared with the normal group,the cell viability of the high glucose group was significantly increased(P<0.05),FN protein expression and PI3 K/Akt phosphorylation level in mesangial cells were significantly increased(P<0.05).Compared with the high glucose group,the cell viability in the 90μmol·L-1 Esculin group was significantly decreased(P<0.05),and FN protein expression and the phosphorylation level of PI3 K/Akt were also significantly decreased(P<0.05).Conclusion Esculin could inhibit cell proliferation and the protein expression of fibronectin in rat mesangial cells induced by high glucose,and its mechanism might be related to suppression of PI3 K/Akt signaling pathway.

关 键 词：秦皮甲素 糖尿病肾病 大鼠肾小球系膜细胞 纤维连接蛋白 PI3K/AKT信号通路 

分 类 号：R285.5[医药卫生—中药学]