SOAT1在肝细胞癌组织中的表达及其临床意义  

Expression of SOAT1 and its clinical significance in hepatocellular carcinoma

作　　者：陈瑶[1,2] 杨训俊 陈涵斌[2] 陈国荣[1,2] CHEN Yao;YANG Xunjun;CHEN Hanbin;CHEN Guorong(Wenzhou Medical University,Wenzhou 325035,China;Department of Pathology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Center of Laboratory Medicine,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China)

机构地区：[1]温州医科大学,浙江温州325035 [2]温州医科大学附属第一医院病理科,浙江温州325015 [3]温州医科大学附属第二医院临床检验中心,浙江温州325027

出　　处：《温州医科大学学报》2021年第1期35-39,共5页Journal of Wenzhou Medical University

基　　金：温州市公益性科技计划项目(Y20190206)。

摘　　要：目的:观察甾醇O-酰基转移酶1(SOAT1)在肝细胞癌(HCC)组织及癌旁组织中的表达,探索其与患者预后及临床病理特征之间的相关性。方法:从癌症基因组图谱(TCGA)数据库收集HCC数据集,下载SOAT1 mRNA表达谱及预后资料,比较SOAT1 mRNA在50例正常肝组织与374例HCC组织中的表达,分析表达量与患者预后相关性。收集53例HCC患者石蜡组织标本,采用免疫组织化学方法检测癌和癌旁组织中SOAT1的蛋白表达水平,并分析其与临床病理特征之间的相关性。结果:TCGA数据库数据分析显示,与癌旁正常组织比较,SOAT1在HCC中表达显著升高(P<0.01)。预后生存分析发现SOAT1高表达患者的5年生存率较低(P<0.05)。免疫组织化学结果显示,与癌旁组织相比,HCC组织中SOAT1蛋白表达量显著升高(P<0.05)。癌组织中SOAT1蛋白表达量与肿瘤临床分期及微血管癌栓形成相关(P<0.05)。结论:SOAT1在HCC组织中表达增高,并与HCC癌患者的临床病理特征密切相关,其表达与肿瘤恶性程度高度相关。Objective:To investigate the expression of sterol O-acyltransferase 1(SOAT1)in hepatocellular carcinoma(HCC)tissues and tissues adjacent to the cancer,and to explore its correlation with patient’s prognosis and clinicopathological features.Methods:The HCC dataset was collected from the Cancer Genome Atlas(TCGA)database,and download the expression of SOAT1 mRNA and prognostic data.The expression of SOAT1 mRNA in 50 normal liver tissues and 374 HCC tissues was compared to analyze the correlation between expression and prognosis.Collected paraffin tissue specimens of 53 HCC patients from the First Affiliated Hospital of Wenzhou Medical University from May 2018 to October 2019,using immunohistochemical(IHC)methods to detect the protein expression level of SOAT1 in cancer and adjacent tissues,and its correlation with clinical pathology features was analyzed.Results:Data analysis of TCGA database showed that compared with normal tissues adjacent to cancer,SOAT1 expression was significantly increased in HCC(P<0.001).The prognostic survival analysis found that 5-year survival rate of high expression of SOAT1 was lower(P<0.05).IHC results showed that compared with adjacent tissues,the expression of SOAT1 protein in HCC tissues was significantly increased(P<0.05).The expression of SOAT1 protein in cancer tissues was correlated with the clinical stage of the tumor and microvascular tumor thrombosis(P<0.05).Conclusion:The expression of SOAT1 is increased in HCC tissues,and closely related to the clinicopathological characteristics of HCC cancer patients,with its expression highly correlated with the malignancy degree of the tumor.

关 键 词：肝细胞癌 甾醇O-酰基转移酶 肿瘤转移 预后分析 

分 类 号：R656[医药卫生—外科学]