基于铕纳米荧光配合物羧基活化原理的手印显现方法  

Europium Nanocomplex for Development of Latent Fingerprints Based on Carboxyl Activation Mechanism

作　　者：朱中旭 王猛[1,2] 李明[1,2] 袁传军 吴建 ZHU Zhong-Xu;WANG Meng;LI Ming;YUAN Chuan-Jun;WU Jian(College of Forensic Sciences,Criminal Investigation Police University of China,Shenyang 110035,China;Research Centre of Crime Governance in the New Era,Criminal Investigation Police University of China,Shenyang 110035,China;Ningbo Institute of Materials Technology and Engineering,Chinese Academy of Sciences,Ningbo 315201,China)

机构地区：[1]中国刑事警察学院刑事科学技术学院,沈阳110035 [2]中国刑事警察学院新时代犯罪治理研究中心,沈阳110035 [3]中国科学院宁波材料技术与工程研究所,宁波315201

出　　处：《分析化学》2021年第2期237-245,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21205139,21802169);公安部科技强警基础工作专项(Nos.2014GABJC033,2018GABJC07,2018GABJC09);辽宁省高等学校创新人才支持计划项目(No.LR2017055);中国刑事警察学院2020年度研究生创新能力提升项目(No.2020YCYB29)资助。

摘　　要：基于羧基活化原理,建立了手印纳米荧光显现方法,其显现效果和显现效率均优于传统化学键合手印显现方法。以铕离子(Eu 3+)为发光中心、对苯二甲酸(PTA)为第一配体、邻菲罗啉(o-Phen)为第二配体,采用化学方法一步合成出表面羧基修饰的铕纳米荧光配合物。使用透射电子显微镜、X射线衍射、紫外可见吸收光谱、荧光光谱、傅里叶变换红外光谱、热重-差示扫描量热分析等技术对纳米配合物的微观形貌、结晶性能、吸收性质、荧光性能、表面基团和热学性质等进行了表征。利用1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)联合N-羟基琥珀酰亚胺(NHS)对纳米配合物表面羧基进行活化,使活化羧基与手印物质中胺基迅速发生酰胺反应,进而将纳米配合物与手印物质相偶联,最终实现光滑非渗透性客体表面潜在手印的高质量和高效率显现。对手印显现机理进行了深入讨论,对纳米配合物显现试剂的组成及手印显现时间进行了系统优化,并从对比度、灵敏度、选择性等角度全面考察了手印显现的效果。An activated carboxyl-based approach for latent fingerprint development with high quality and high efficiency was proposed. Firstly, carboxyl-functionalized fluorescent europium nanocomplex was chemically synthesized via a one-step process by using europium (Ⅲ) ion as the luminescence center, phthalic acid as the first ligand, and phenanthroline as the second ligand. Then, the micromorphology, crystal structure, ultraviolet absorption property, fluorescent emission performance, surface functional groups and thermal property of these synthesized europium nanocomplex were characterized by transmission electron microscopy, powder X-ray diffractometer, ultraviolet-visible spectrophotometer, fluorescence spectrophotometer, Fourier transform infrared spectrometer and simultaneous thermal analyzer, respectively. The carboxyl groups on the surface of europium nanocomplex were further activated using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride combined with N-hydroxysuccinimide. The activated carboxyl groups on the surface of europium nanocomplex could easily react with the amino groups in latent fingerprint through amide reaction, and the latent fingerprint was thus fluorescently developed with high efficiency. The mechanism of latent fingerprint development based on activated carboxyl process was discussed in detail, and the nanocomplex dosage and the staining time in developing suspension were also systematically optimized. In addition, the effects in fingerprint development were investigated from three aspects including contrast, sensitivity and selectivity. Experimental results showed that, the activated carboxyl-based approach could achieve a high performance in latent fingerprint development, exhibiting high efficiency, good operability and wide applicability.

关 键 词：手印 显现 稀土 荧光 纳米材料 铕配合物 

分 类 号：O641.4[理学—物理化学] TB383.1[理学—化学] D918.91[一般工业技术—材料科学与工程]