基因枪介导抗草甘膦基因对玉米自交系A188的遗传转化  

Genetic transformation of maize inbred line A188 with the glyphosate-resistant gene mediated by particle bombardment

作　　者：韩平安 吴新荣 孙瑞芬 常悦 石海波 唐宽刚 梁亚晖 李晓东 HAN Ping′an;WU Xinrong;SUN Ruifen;CHANG Yue;SHI Haibo;TANG Kuangang;LIANG Yahui;LI Xiaodong(Biotechnology Research Center,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Maize Research Institute,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古自治区农牧业科学院生物技术研究中心,内蒙古呼和浩特010031 [2]内蒙古自治区农牧业科学院玉米研究所,内蒙古呼和浩特010031

出　　处：《北方农业学报》2020年第6期1-5,共5页Journal of Northern Agriculture

基　　金：内蒙古农牧业创新基金项目(2018CXJJN06,2015CXJJN04)。

摘　　要：【目的】有效利用除草剂进行杂草防治,培育抗除草剂转基因玉米种质材料,进一步完善玉米转基因育种技术。【方法】采用基因枪对玉米自交系A188幼胚愈伤组织进行遗传转化研究,实施单因素试验,分别设置不同胚的大小、除草剂浓度,比较分析愈伤组织诱导率。【结果】玉米幼胚大小为1.5~2.0 mm时,愈伤组织的诱导率最高(93.3%);双丙氨膦浓度为3 mg/L时是除草剂最佳筛选浓度;PCR检测证明得到5株转基因苗,同时转基因苗抗性鉴定试验表现出较强草甘膦抗性。【结论】建立基因枪介导的玉米遗传转化体系,为培育抗除草剂玉米种质材料奠定了基础。【Objective】In order to effectively use herbicides for weed control,the herbicide resistant transgenic maize germplasm materials were cultivated,and the transgenic maize breeding technology was further improved.【Methods】Genetic transformation was conducted on immature embryos of callus induction of maize inbred line A188 by particle bombardment,which was carried out single factor test,the different size of immature embryo and concentration of herbicide were set respectively,and the callus induction rate was compared and analyzed.【Results】The results showed that the callus induction rate was the highest(93.3%)when the size of the immature embryo was 1.5-2.0 mm.When the concentration of bialaphos was 3 mg/L,it was the best screening concentration of herbicide.PCR detection proved that 5 positive transgenic seedlings were successfully obtained,and they showed strong glyphosate resistance in transgenic seedlings of resistance identification tests.【Conclusion】This study provides a reference for the establishment of maize genetic transformation system mediated by particle bombardment and for the cultivation of herbicide-resistant maize germplasm materials.

关 键 词：玉米 抗草甘膦基因(EPSPS) 基因枪介导 遗传转化 

分 类 号：S513[农业科学—作物学]