Systematic assessment of Pichia pastoris system for optimized b -galactosidase production  

作　　者：Hongbing Sun Olufemi Emmanuel Bankefa Ijeoma Onyinyechi Ijeoma Liangtian Miao Taicheng Zhu Yin Li 

机构地区：[1]CAS Key Laboratory of Microbial Physiological and Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing,100101,China [2]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,300308,China [3]Department of Microbiology,University of Port Harcourt,Port Harcourt,Nigeria

出　　处：《Synthetic and Systems Biotechnology》2017年第2期113-120,共8页合成和系统生物技术（英文）

基　　金：This work was supported by Key International Cooperation Project from Chinese Academy of Sciences(155112KYSB20160010);Beijing Municipal Natural Science Foundation(5132024);National Natural Science Foundation of China(31000026).

摘　　要：1.Introduction b-Galactosidase(commonly known as b-lactase;EC 3.2.1.23)is a multifunctional enzyme that can catalyze the hydrolysis of terminal non-reducing b-D-galactose residues in b-D-galactosides or transfer the galactosyl residue to saccharide acceptors to yield galactooligosaccharides(GOS).b-Galactosidase has a variety of applications in food and medical industries such as hydrolysis of lactose in milk,manufacture of galactooligosaccharides(GOS)and treatment of lactose malabsorption[1].Although b-galactosidase is an ubiquitous enzyme existing in plants,animals and microorganisms,only a few b-galactosidases from Kluveromyces lactis,Aspergillus niger and Aspergillus oryzae are regarded as safe for food related industry applications.

关 键 词：HYDROLYSIS SYSTEM RESIDUE 

分 类 号：F42[经济管理—产业经济]