Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of Schizochytrium sp.and Thraustochytriidae sp.through bioprocess optimization  

作　　者：Qiuzhen Wang Huike Ye Biswarup Sen Yunxuan Xie Yaodong He Sunghoon Park Guangyi Wang 

机构地区：[1]Center for Marine Environmental Ecology,School of Environmental Science and Engineering,Tianjin University,Tianjin,300072,China [2]Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin,300072,China [3]School of Energy and Chemical Engineering,UNIST,Ulsan,South Korea

出　　处：《Synthetic and Systems Biotechnology》2018年第2期121-129,共9页合成和系统生物技术（英文）

基　　金：This work was partially supported by National Key R&D Program of China(2016YFA0601400);National Science Foundation of China(31670044,91751115,and 31602185).

摘　　要：Thraustochytrids,rich in docosahexaenoic acid(DHA,C22:6u3),represent a potential source of dietary fatty acids.Yet,the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains.Two different thraustochytrid strains,Schizochytrium sp.PKU#Mn4 and Thraustochytriidae sp.PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions.Although they exhibited similar fatty acid profiles,PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions.Relative DHA content of 48.5%and 49.2%(relative to total fatty acids),respectively,were achieved on glycerol under their optimal flask culture conditions.Maximum DHA yield(Yp/x)of 21.0%and 18.9%and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained,respectively,in 5-L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy.A 3.4-and 2.8-fold improvement of DHA production(g/L),respectively,was achieved in this study.Overall,our study provides the potential of two thraustochytrid strains and their culture conditions for efficient production of DHA-rich oil.

关 键 词：THRAUSTOCHYTRIDS GLYCEROL Polyunsaturated fatty acids Docosahexaenoic acids Batch culture 

分 类 号：F42[经济管理—产业经济]