Discovery and modification of cytochrome P450 for plant natural products biosynthesis  

作　　者：Xiaonan Liu Xiaoxi Zhu Hui Wang Tian Liu Jian Cheng Huifeng Jiang 

机构地区：[1]Key Laboratory of Systems Microbial Biotechnology,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,300308,China [2]University of Chinese Academy of Sciences,Beijing,100049,China [3]College of Biotechnology,Tianjin University of Science&Technology,Tianjin,300457,China [4]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning,Guangxi,530004,China

出　　处：《Synthetic and Systems Biotechnology》2020年第3期187-199,共13页合成和系统生物技术（英文）

基　　金：This work was supported by grants from the National Key R&D Program of China(No.2019YFA0905700)to J.C.;the National Natural Science Foundation of China(NSFC;Grant No.31901026);the China Postdoctoral Science Foundation(Grant No.2019M661032)to X.L;Tianjin Science and technology plan project(Grant No.19PTZWHZ00060);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-007&TSBICIP-KJGG-002)to H.J.

摘　　要：Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products.Over the last few years,there has been remarkable progress in plant P450s identification with the rapid development of sequencing technology,“omics”analysis and synthetic biology.However,challenges still persist in respect of crystal structure,heterologous expression and enzyme engineering.Here,we reviewed several research hotspots of P450 enzymes involved in the biosynthesis of plant natural products,including P450 databases,gene mining,heterologous expression and protein engineering.

关 键 词：P450 enzymes Plant natural products Synthetic biology 

分 类 号：Q943.2[生物学—植物学]