Interrogation of Streptomyces avermitilis for efficient production of avermectins  

作　　者：Jinsong Chen Mei Liu Xueting Liu Jin Miao Chengzhang Fu Heyong Gao Rolf Muller Qing Zhang Lixin Zhang 

机构地区：[1]Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China [2]Graduate University of Chinese Academy of Sciences,Beijing,China [3]Helmholtz Institute for Pharmaceutical Research Saarland,Helmholtz Centre for Infection Research,66123,Saarbrucken,Germany [4]Department of Pharmaceutical Biotechnology,Saarland University,Building C 2.3,66123,Saarbrucken,Germany [5]Inner Mongolia New Veyong Biochemical Co.,Ltd,Inner Mongolia Dalad Banner,China

出　　处：《Synthetic and Systems Biotechnology》2016年第1期7-16,共10页合成和系统生物技术（英文）

基　　金：the National Program on Key Basic Research Project(973 program,2013CB734000 and 2012CB725203);the National Natural Science Foundation of China(31170095,31000057,and 31100075);the Ministry of Science and Technology of the People’s Republic of China(2011ZX11102-011-11 and 2013ZX10005004-005);China Ocean Mineral Resources R&D Association(DY125-15-T-07)。

摘　　要：The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin,respectively.Avermectins produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic agents.Because wild-type strains only produce low levels of avermectins,much research effort has focused on improvements in avermectin production to meet the ever increasing demand for such compounds.This review describes the strategies that have been widely employed and the future prospects of synthetic biology applications in avermectin yield improvement.With the help of genome sequencing of S.avermitilis and an understanding of the avermectin biosynthetic/regulatory pathways,synthetic and systems biotechnology approaches have been applied for precision engineering.We focus on the design and synthesis of biological chassis,parts,devices,and modules from diverse microbes to reconstruct and optimize their dynamic processes,as well as predict favorable effective overproduction of avermectins by a 4Ms strategy(Mine,Model,Manipulation,and Measurement).

关 键 词：AVERMECTINS Metabolic engineering Streptomyces avermitilis Synthetic biology 

分 类 号：S48[农业科学—农药学]