Genome mining reveals the biosynthetic potential of the marinederived strain Streptomyces marokkonensis M10  

作　　者：Liangyu Chen Ying-Mi Lai Yu-Liang Yang Xinqing Zhao 

机构地区：[1]School of Life Science and Biotechnology,Dalian University of Technology,Dalian 116024,China [2]Agricultural Biotechnology Research Center,Academia Sinica,Taipei 11529,Taiwan [3]State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Synthetic and Systems Biotechnology》2016年第1期56-65,共10页合成和系统生物技术（英文）

摘　　要：Marine streptomycetes are rich sources of natural products with novel structures and interesting biological activities,and genome mining of marine streptomycetes facilitates rapid discovery of their useful products.In this study,a marine-derived Streptomyces sp.M10 was revealed to share a 99.02%16S rDNA sequence identity with that of Streptomyces marokkonensis Ap1T,and was thus named S.marokkonensis M10.To further evaluate its biosynthetic potential,the 7,207,169 bps of S.marokkonensis M10 genome was sequenced.Genomic sequence analysis for potential secondary metaboliteassociated gene clusters led to the identification of at least three polyketide synthases(PKSs),six non-ribosomal peptide synthases(NRPSs),one hybrid NRPS-PKS,two lantibiotic and five terpene biosynthetic gene clusters.One type I PKS gene cluster was revealed to share high nucleotide similarity with the candicidin/FR008 gene cluster,indicating the capacity of this microorganism to produce polyene macrolides.This assumption was further verified by isolation of two polyene family compounds PF1 and PF2,which have the characteristic UV adsorption at 269,278,290 nm(PF1)and 363,386 and 408 nm(PF2),respectively.S.marokkonensis M10 is therefore a new source of polyene metabolites.Further studies on S.marokkonensis M10 will provide more insights into natural product biosynthesis potential of related streptomycetes.This is also the first report to describe the genome sequence of S.marokkonensis-related strain.

关 键 词：Streptomyces marokkonensis Genome mining Secondary metabolites Polyketide synthase Polyene macrolides 

分 类 号：O63[理学—高分子化学]