Engineering bacteria for enhanced polyhydroxyalkanoates(PHA)biosynthesis  

作　　者：Guo-Qiang Chen Xiao-Ran Jiang 

机构地区：[1]School of Life Sciences,Tsinghua University,Beijing 100084,China [2]Center for Synthetic and Systems Biology,Tsinghua University,Beijing 100084,China [3]Tsinghua-Peking Center for Life Sciences,Tsinghua University,Beijing 100084,China [4]Center for Nano and Micro Mechanics,Tsinghua University,Beijing 100084,China [5]MOE Key Lab of Industrial Biocatalysis,Dept Chemical Engineering,Tsinghua University,Beijing 100084,China

出　　处：《Synthetic and Systems Biotechnology》2017年第3期192-197,共6页合成和系统生物技术（英文）

基　　金：This research was financially supported by a grant from Ministry of Sciences and Technology(Grant No.2016YFB0302504);grants from National Natural Science Foundation of China(Grant No.31430003);Tsinghua President Fund also supported this project(Grant No.2015THZ10).

摘　　要：Polyhydroxyalkanoates(PHA)have been produced by some bacteria as bioplastics for many years.Yet their commercialization is still on the way.A few issues are related to the difficulty of PHA commercialization:namely,high cost and instabilities on molecular weights(Mw)and structures,thus instability on thermo-mechanical properties.The high cost is the result of complicated bioprocessing associated with sterilization,low conversion of carbon substrates to PHA products,and slow growth of microorganisms as well as difficulty of downstream separation.Future engineering on PHA producing microorganisms should be focused on contamination resistant bacteria especially extremophiles,developments of engineering approaches for the extremophiles,increase on carbon substrates to PHA conversion and controlling Mw of PHA.The concept proof studies could still be conducted on E.coli or Pseudomonas spp.that are easily used for molecular manipulations.In this review,we will use E.coli and halophiles as examples to show how to engineer bacteria for enhanced PHA biosynthesis and for increasing PHA competitiveness.

关 键 词：PHB POLYHYDROXYALKANOATES EXTREMOPHILES HALOPHILES Next generation industrial biotechnology NGIB Metabolic engineering Pathway engineering Morphology engineering Contents 

分 类 号：Q93[生物学—微生物学]