Evaluation of fermentation conditions triggering increased antibacterial activity from a near-shore marine intertidal environment-associated Streptomyces species  

作　　者：A.L.English A.Boufridi R.J.Quinn D.I.Kurtboke 

机构地区：[1]Genecology Research Centre and Faculty of Science,Health,Education and Engineering,University of the Sunshine Coast,Maroochydore DC,QLD,4558,Australia [2]Eskitis Institute for Drug Discovery,Griffith University,Nathan,QLD,4111,Australia

出　　处：《Synthetic and Systems Biotechnology》2017年第1期28-38,共11页合成和系统生物技术（英文）

摘　　要：A near-shore marine intertidal environment-associated Streptomyces isolate(USC-633)from the Sunshine Coast Region of Queensland,Australia,cultivated under a range of chemically defined and complex media to determine optimal parameters resulting in the secretion of diverse array of secondary metabolites with antimicrobial properties against various antibiotic resistant bacteria.Following extraction,fractioning and re-testing of active metabolites resulted in persistent antibacterial activity against Escherichia coli(Migula)(ATCC 13706)and subsequent Nuclear Magnetic Resonance(NMR)analysis of the active fractions confirmed the induction of metabolites different than the ones in fractions which did not display activity against the same bacterial species.Overall findings again confirmed the value of One StraineMany Compounds(OSMAC)approach that tests a wide range of growth parameters to trigger bioactive compound secretion increasing the likelihood of finding novel therapeutic agents.The isolate was found to be adaptable to both marine and terrestrial conditions corresponding to its original nearshore marine intertidal environment.Wide variations in its morphology,sporulation and diffusible pigment production were observed when different growth media were used.

关 键 词：Marine streptomycetes Marine biodiscovery Fermentation media ANTIBIOTICS Marine natural products PIGMENTS 

分 类 号：P73[天文地球—海洋科学]