Rational and semi-rational engineering of cytochrome P450s for biotechnological applications  

作　　者：Lian-Hua Xu Yi-Ling Du 

机构地区：[1]College of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China [2]Institute of Pharmaceutical Biotechnology,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China

出　　处：《Synthetic and Systems Biotechnology》2018年第4期283-290,共8页合成和系统生物技术（英文）

基　　金：fundamental research funds from the National Natural Science Foundation(grant number 81402810)。

摘　　要：The cytochrome P450 enzymes are ubiquitous heme-thiolate proteins performing regioselective and stereoselective oxygenation reactions in cellular metabolism.Due to their broad substrate scope and catalytic versatility,P450 enzymes are also attractive candidates for many industrial and biopharmaceutical applications.For particular uses,enzyme properties of P450s can be further optimized through directed evolution,rational,and semi-rational engineering approaches,all of which introduce mutations within the P450 structures.In this review,we describe the recent applications of these P450 engineering approaches and highlight the key regions and residues that have been identified using such approaches.These“hotspots”lie within critical functional areas of the P450 structure,including the active site,the substrate access channel,and the redox partner interaction interface.

关 键 词：Cytochrome P450 Protein engineering Rational design Crystal structure 

分 类 号：O64[理学—物理化学]