The road less traveled:A new phosphorothioate antiviral defense mechanism discovered in Archaea  

作　　者：Michael S.DeMott Peter C.Dedon 

机构地区：[1]Department of Biological Engineering,Massachusetts Institute of Technology,Cambridge,MA 02139,USA [2]Antimicrobial Resistance Interdisciplinary Research Group,Singapore-MIT Alliance for Research and Technology,138602,Singapore

出　　处：《Synthetic and Systems Biotechnology》2019年第3期132-133,共2页合成和系统生物技术（英文）

基　　金：the National Science Foundation of the USA(CHE-1709364);the National Research Foundation of Singapore in the Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance IRG.

摘　　要：The story of phosphorothioate modifications of DNA begins over 50 years ago and has unfolded like Frost's walk in the woods[1].In 1970,Fritz Eckstein synthesized oligonucleotides in which a non-bridging oxygen in the phosphodiester backbone was replaced by sulfur–the first phosphorothioate-modified nucleic acid[2].Twenty years passed until Zixin Deng and colleagues discovered a DNA degradation phenotype in bacteria possessing a 5-gene dnd cluster,with electrophoretic degradation caused by incorporation of sulfur into bacterial genomes[3].These roads of chemistry and biology met again another 20 years later with the discovery by Wang et al.that Dnd proteins insert sulfur as a phosphorothioate in the genomes of diverse bacteria[4].This walk in the woods now picked up speed.

关 键 词：SULFUR BACKBONE BRIDGING 

分 类 号：O62[理学—有机化学]