Detailed profiling of carbon fixation of in silico synthetic autotrophy with reductive tricarboxylic acid cycle and Calvin-Benson-Bassham cycle in Esherichia coli using hydrogen as an energy source  

作　　者：Hsieh-Ting-Yang Cheng Shou-Chen Lo Chieh-Chen Huang Tsung-Yi Ho Ya-Tang Yang 

机构地区：[1]Department of Computer Science,National Tsing Hua University,Hsinchu,30013,Taiwan,R.O.C [2]Department of Life Sciences,National Chung-Hsing Univeristy,Taichung,Taiwan,R.O.C [3]Department of Electrical Engineering,National Tsing Hua University,Hsinchu,30013,Taiwan,R.O.C [4]Program in Microbial Genomics,National Chung Hsing University,Taichung,Taiwan,R.O.C [5]Innovation and Development Center of Sustainable Agriculture,National Chung Hsing University,Taichung,Taiwan,R.O.C

出　　处：《Synthetic and Systems Biotechnology》2019年第3期165-172,共8页合成和系统生物技术（英文）

基　　金：Y.T.Y.would like to acknowledge funding support from the Ministry of Science and Technology under grant numbers MOST 105-2221-E-007-130-MY3 and MOST 107-2621-M-007-001-MY3;C.C.H.would like to acknowledge funding support from the Ministry of Science and Technology under grant numbers MOST 107-2621-M-005-007-MY3 and 107-2621-M-005-001.

摘　　要：Carbon fixation is the main route of inorganic carbon in the form of CO2 into the biosphere.In nature,RuBisCO is the most abundant protein that photosynthetic organisms use to fix CO2 from the atmosphere through the Calvin-Benson-Bassham(CBB)cycle.However,the CBB cycle is limited by its low catalytic rate and low energy efficiency.In this work,we attempt to integrate the reductive tricarboxylic acid and CBB cycles in silico to further improve carbon fixation capacity.Key heterologous enzymes,mostly carboxylating enzymes,are inserted into the Esherichia coli core metabolic network to assimilate CO2 into biomass using hydrogen as energy source.Overall,such a strain shows enhanced growth yield with simultaneous running of dual carbon fixation cycles.Our key results include the following.(i)We identified two main growth states:carbon-limited and hydrogenlimited;(ii)we identified a hierarchy of carbon fixation usage when hydrogen supply is limited;and(iii)we identified the alternative sub-optimal growth mode while performing genetic perturbation.The results and modeling approach can guide bioengineering projects toward optimal production using such a strain as a microbial cell factory.

关 键 词：Reductive tricarboxylic acid cycle Calvin-Benson-Bassham cycle Carbon fixation Metabolism 

分 类 号：O64[理学—物理化学]