Methodologies for preparation of prokaryotic extracts for cell-free expression systems  

作　　者：Stephanie D.Cole Aleksandr E.Miklos Abel C.Chiao Zachary Z.Sun Matthew W.Lux 

机构地区：[1]US Army Combat Capabilities Development Command Chemical Biological Center,8567 Ricketts Point Road,Aberdeen Proving Ground,MD,21010,USA [2]Division of Biology and Biological Engineering,California Institute of Technology,Pasadena,CA,USA [3]Synvitrobio Inc.,San Francisco,CA,USA [4]8567 Ricketts Point Road,Bldg E3549 Room B140,APG,MD,21010,USA

出　　处：《Synthetic and Systems Biotechnology》2020年第4期252-267,共16页合成和系统生物技术（英文）

摘　　要：Cell-free systems that mimic essential cell functions,such as gene expression,have dramatically expanded in recent years,both in terms of applications and widespread adoption.Here we provide a review of cell-extract methods,with a specific focus on prokaryotic systems.Firstly,we describe the diversity of Escherichia coli genetic strains available and their corresponding utility.We then trace the history of cell-extract methodology over the past 20 years,showing key improvements that lower the entry level for new researchers.Next,we survey the rise of new prokaryotic cell-free systems,with associated methods,and the opportunities provided.Finally,we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.

关 键 词：Cell-free systems Cell-free expression Cell-free extract Synthetic biology Methods 

分 类 号：Q81[生物学—生物工程]