Molecular characterization and antibacterial effect of endophytic actinomycetes Nocardiopsis sp. GRG1 (KT235640) from brown algae against MDR strains of uropathogens  

作　　者：Govindan Rajivgandhi Ramachandran Vijayan Marikani Kannan Malairaja Santhanakrishnan Natesan Manoharan 

机构地区：[1]Department of Marine Science,Bharathidasan University,Tiruchirappalli 24,Tamil Nadu,India [2]School of Life Sciences,Jawaharlal Nehru University,New Delhi 67,India [3]Department of Microbiology,VHNSN College,Virudunagar 01,Tamil Nadu,India [4]Department of Marine and Coastal Studies,Madurai Kamaraj University,Madurai 21,Tamil Nadu,India

出　　处：《Bioactive Materials》2016年第2期140-150,共11页生物活性材料（英文）

基　　金：We gratitude to the Bharathidasan University,Tiruchirappalli-24 for University Research Fellowship(URF)(Ref.No.05441/URF/K7/2013).

摘　　要：Our study is to evaluate the potential bioactive compound of Nocardiopsis sp.GRG1(KT235640)and its antibacterial activity against multi drug resistant strains(MDRS)on urinary tract infections(UTIs).Two brown algae samples were collected and were subjected to isolation of endophytic actinomycetes.100 strains of actinomycetes were isolated from algal samples based on observation of morphology and physiological characters.40 strains were active in antagonistic activity against various clinical pathogens.Among the strains,10 showed better antimicrobial activity against MDRS on UTIs.The secondary metabolite of Nocardiopsis sp.GRG1(KT235640)has showed tremendous antibacterial activity against UTI pathogens compared to other strains.Influence of various growth parameters were used for synthesis of secondary metabolites,such as optimum pH 7,incubation time 5e7 days,temperature(30
C),salinity(5%),fructose and mannitol as the suitable carbon and nitrogen sources.At 100 μg/ml concentration MIC of Nocardiopsis sp.GRG1(KT235640)showed highest percentage of inhibition against Proteus mirabilis(85%),and E.coli,Staphylococcus auerues,Psuedomonas aeroginasa,Enterobactor sp and Coagulinase negative staphylococci 78e85%respectively.

关 键 词：ENDOPHYTES Multi-drug resistant strains Urinary tract infections Minimal inhibitory concentration 

分 类 号：Q93[生物学—微生物学]