Bioactivation of titanium dioxide scaffolds by ALP-functionalization  

作　　者：A.Sengottuvelan P.Balasubramanian J.Will A.R.Boccaccini 

机构地区：[1]Department of Materials Science and Engineering,Institute of Biomaterials,University of Erlangen-Nuremberg,Cauerstraße 6,91058 Erlangen,Germany

出　　处：《Bioactive Materials》2017年第2期108-115,共8页生物活性材料（英文）

基　　金：The authors would like to acknowledge Dr.Tobias Fey(University of Erlangen-Nuremberg)for helping with XRD measurements;Mr.Marvin Valley(Institute of Biomaterials,University of Erlangen-Nuremberg)for experimental support.

摘　　要：Three dimensional TiO2 scaffolds are receiving renewed attention for bone tissue engineering (TE) due totheir biocompatibility and attractive mechanical properties. However the bioactivity of these scaffolds iscomparatively lower than that of bioactive glass or hydroxyapatite (HA) scaffolds. One strategy toimprove bioactivity is to functionalize the surface of the scaffolds using biomolecules. Alkaline phosphatase(ALP) was chosen in this study due to its important role in the bone mineralization process. Thecurrent study investigated the ALP functionalization of 3D titanium dioxide scaffolds using selfpolymerizationof dopamine. Robust titanium scaffolds (compressive strength^2.7 ± 0.3 MPa) wereproduced via foam replica method. Enzyme grafting was performed by dip-coating in polydopamine/ALPsolution. The presence of ALP was indirectly confirmed by contact angle measurements and enzymaticactivity study. The influence of the enzyme on the bioactivity, e.g. hydroxyapatite formation on thescaffold surface, was measured in simulated body fluid (SBF). After 28 days in SBF, 5 mg ALP coatedtitania scaffolds exhibited increased hydroxyapatite formation. It was thus confirmed that ALP enhancesthe bioactivity of titania scaffolds, converting an inert bioceramic in an attractive bioactive system forbone TE.

关 键 词：Alkaline phosphatase Titanium dioxide Scaffolds POLYDOPAMINE Surface modification Bioactivity 

分 类 号：R31[医药卫生—基础医学]