表面等离子体共振传感技术应用于小分子检测  

Surface Plasmon Resonance Sensing Technology is Applied to Small Molecule Detection

作　　者：刘雪梅 王晓琳 仇增凤 王亚东 张斌 徐超[1] 尹洪宗[1] LIU Xue-mei;WANG Xiao-lin;QIU Zeng-feng;WANG Ya-dong;ZHANG Bin;XU Chao;YIN Hong-zong(College of Chemistry and Materials Science,Shandong Agricultural University,Tai’an271018,China)

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018

出　　处：《光谱学与光谱分析》2021年第2期511-516,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21802089);山东省自然科学基金项目(ZR2019BB015)资助。

摘　　要：人体内的生物小分子对维持生命体健康具有重要影响,此类分子参与生命体的血液循环及免疫系统,因此检测生命体中生物小分子对研究小分子在生命体中的生理功能有着重要的意义。傅里叶变换-表面等离子体共振(Fourier transform-surface plasmon resonance,FT-SPR)光谱技术具有操作简便、灵敏度高、所需样品少、可实时检测等优点,近年越来越多应用于分子的检测。对于部分分子尤其是分子量非常小的生物小分子,FT-SPR直接检测法存在信号稳定性差、灵敏度不高等缺点,因此可以针对不同的检测目标构建系列SPR小分子生物传感器通过信号扩增弥补以上缺点。该研究分类并列举了用于小分子检测的SPR免疫传感器(直接检测法、“三明治”法、竞争检测法以及抑制检测法)、SPR分子印迹传感器以及其他基于SPR传感器,系统综述了SPR技术检测小分子的研究进展,并对其发展远景进行了展望。Small biomolecules in the human body have important effects on the health of the living body.Some of them are involved in the blood circulation and immune system of the living body Therefore,the detection of small molecules in living organisms in the study of small molecules in the physiological function of life is of great significance.Fourier Transform Surface Plasmon Resonance(FT-SPR)spectroscopy has been widely used in molecular detection in recent years due to its advantages of simple operation,high sensitivity,few samples required and real-time detection But for different molecules,especially those with very small molecular weight,FT-SPR direct detection has some disadvantages,such as unstable signal and low sensitivity.Therefore,a series of SPR small molecule sensors can be constructed for different detection targets to remedy the above shortcomings.In this paper,the research progress in the detection of small molecules by SPR technology is reviewed from the aspects of immunosensor(direct detection,Sandwich detection,competitive detection and inhibition detection),SPR molecular imprinting sensor and other SPR based sensors.The prospect of its development is also given.

关 键 词：表面等离子体共振技术 小分子检测 表面等离子体传感器 

分 类 号：O657.3[理学—分析化学]