高效液相色谱–波长切换法同时测定独一味软胶囊中6种成分  

Simultaneous determination of six components in duyiwei soft capsulesby by HPLC-wavelength switching

作　　者：周颖 昝珂[2] 郑成 Zhou Ying;Zan Ke;Zheng Cheng(Zhejiang Institute for Food and Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine),Hangzhou 310052,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]浙江省食品药品检验研究院,国家药品监督管理局中成药质量评价重点实验室,杭州310052 [2]中国食品药品检定研究院,北京100050

出　　处：《化学分析计量》2021年第2期56-60,共5页Chemical Analysis And Meterage

摘　　要：建立高效液相色谱–波长切换法同时测定独一味软胶囊中山栀苷甲酯、绿原酸、8-O-乙酰山栀苷甲酯、连翘酯苷B、毛蕊花糖苷和木犀草苷的方法。以Symmetry Shield RP18柱(250 mm×4.6 mm,5μm)为色谱柱,乙腈–0.3%磷酸水为流动相,检测波长分别为235 nm(山栀苷甲酯、8-O-乙酰山栀苷甲酯)、330 nm(绿原酸、连翘酯苷B、毛蕊花糖苷)、360 nm(木犀草苷),流量为1 mL/min。山栀苷甲酯、绿原酸、8-O-乙酰山栀苷甲酯、连翘酯苷B、毛蕊花糖苷和木犀草苷的进样量在各自的范围内与色谱峰面积线性关系良好,相关系数不小于0.9990,测定结果的相对标准偏差为0.21%~0.69%(n=6),平均加标回收率为94.0%~101.1%。该方法简单、准确、重现性好,适用于独一味软胶囊的质量评价。A method of simultaneously determining menthyl chloride,chlorogenic acid,8-O-acetyl-phloridzin methyl ester,forsythiaside B,ergosterol and luteolin in Duyiwei soft capsules by HPLC–wavelength switching was established.The determination was performed on Symmetry Shield RP18 column(250 mm×4.6 mm,5μm)with mobile phase consisted of acetonitrile–0.3%phosphoric acid solution at the flow rate of 1 mL/min.The detection wavelength were set at 235 nm(menthyl chloride、8-O-acetyl-phloridzin methyl ester),330 nm(chlorogenic acid,forsythiaside B,ergosterol)and 360 nm(luteolin).The injection volume of menthyl chloride,chlorogenic acid,8-O-acetyl-phloridzin methyl ester,forsythiaside B,ergosterol and luteolin had good linear range with chromatographic peak areas,the correlation coefficients were not less than 0.9990.The average recovery was 94.0%–101.1%and the relative standard deviations was 0.21%–0.69%(n=6).The method is simple,scientificly,and reliable for quality control of the Duyiwei soft capsules.

关 键 词：独一味软胶囊 高效液相色谱–波长切换法 山栀苷甲酯 绿原酸 8-O-乙酰山栀苷甲酯 连翘酯苷B 毛蕊花糖苷 木犀草苷 

分 类 号：O657.7[理学—分析化学]