骨内压干预对DH豚鼠退变胫股关节软骨保护作用及软骨内2型胶原与MMP13表达的影响  

The effect of intraosseous pressure intervention on protection of degeneration tibiofemoral joint cartilage of DH guinea pig and the expressions of type 2 collagen and MMP13 in cartilage

作　　者：王硕[1,2] 马剑雄 杜育任[2] 黄洪超 焦尚起[1] 史建国[1] 马信龙 WANG Shuo;MA Jianxiong;DU Yuren;HUANG Hongchao;JIAO Shangqi;SHI Jianguo;MA Xinlong(Department of Orthopaedics,Tianjin Rehabilitaion and Sanatorium Center of People′s Liberation Army/the original PLA NO.464 Hospital,Tianjin 300381,China;Insititute of Orthopaedics,Tianjin Hospital,Tianjin 300050,China)

机构地区：[1]中国人民解放军天津康复疗养中心/原解放军464医院骨科,天津300381 [2]天津医院骨科研究所,300050

出　　处：《重庆医学》2021年第2期181-187,192,共8页Chongqing medicine

基　　金：科技部国家重点研发计划(2016YFC0105805);国家自然科学基金项目(11772226、81572154)。

摘　　要：目的探讨骨内压干预对DH豚鼠退变胫股关节软骨的保护作用及对软骨内2型胶原、基质金属蛋白酶13(MMP13)表达的影响。方法雄性DH豚鼠6、9、12、18月龄各10只分成非减压组(每月龄各5只)及减压组(每月龄各5只);非减压组适应性饲养结束后,测定胫股关节骨内压;减压组行减压术后测定骨内压。实验结束后,实时荧光定量PCR(RT-qPCR)及免疫组织化学法测定胫股关节软骨2型胶原、MMP13 mRNA和蛋白表达水平。结果减压组6、9、12、18月龄豚鼠胫股关节骨内压、MMP13 mRNA和蛋白表达水平均明显低于非减压组(P<0.05);随月龄增长,非减压组、减压组胫股关节骨内压先升后降,在12月龄时达到峰值(P<0.05),MMP13 mRNA和蛋白表达水平逐渐升高,至18月龄时达到峰值(P<0.05)。减压组胫股关节2型胶原mRNA和蛋白表达水平均明显高于非减压组(P<0.05);随月龄增长,非减压组、减压组胫股关节2型胶原mRNA和蛋白表达水平逐渐降低,至18月龄时达到最低值(P<0.05)。结论骨内减压能明显降低DH豚鼠退变胫股关节骨内压,对胫股关节软骨具有保护作用,其机制可能与其促进退变胫股关节2型胶原mRNA和蛋白表达,抑制MMP13 mRNA和蛋白表达相关。Objective To explore the protective effect of intraosseous pressure intervention on the degeneration of tibiofemoral articular cartilage in DH guinea pigs and the effect on the expressions of type 2 collagen and matrix metalloproteinase 13(MMP13)in the cartilage.Methods Forty male DH guinea pigs aged 6,9,12,and 18 months old(10 per each month age)were randomly divided into the non-decompression group(5 per each month age)and the decompression group(5 per each month age).Intraosseous pressure of tibiofemoral joint was measured after adaptive feeding in the non-decompression group,intraosseous pressure was measured after decompression surgery in the decompression group.After the experiment,real-time fluorescent quantitative PCR(RT-qPCR)and immunohistochemistry were used to determine the levels of type 2 collagen,MMP13 mRNA and protein of tibiofemoral articular cartilage.Results The intraosseous pressure,MMP13 mRNA and protein expression levels of tibiofemoral joint of each month-old guinea pig in the decompression group were significantly lower than those in the non-decompression group(P<0.05).With the increase of months,the intraosseous pressure of the tibiofemoral joint in both non-decompression group and decompression group firstly increased and then declined,reaching its peak at 12 months of age(P<0.05),and the expression levels of MMP13 mRNA and protein gradually increased,reaching the peak at 18 months of age(P<0.05).The expression levels of type 2 collagen mRNA and protein of tibiofemoral joint in the decompression group were significantly higher than those in the non-decompression group(P<0.05).With the increase of months,the expression levels of type 2 collagen of tibiofemoral joint in both non-decompression group and decompression group gradually decreased,reaching the lowest value at 18 months of age(P<0.05).Conclusion Intraosseous decompression surgery can significantly reduce the intraosseous pressure of degenerative tibiofemoral joint in DH guinea pigs,and has a protective effect on tibiofemoral artic

关 键 词：骨关节炎 膝 软骨退变 骨内压 软骨内2型胶原 基质金属蛋白酶13 豚鼠 

分 类 号：R684.3[医药卫生—骨科学]